通过Clostridium carboxidivorans进行连续的HBE发酵:CO与合成气相比
F Lanzillo1, S Pisacane1, M Capilla2
1Department of Chemical, Materials and Production Engineering-Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125 Napoli, Italy.
New biotechnology
|February 24, 2024
概括
克洛斯特里碳酸酸能有效地将合成气发酵成酒精. 最佳条件和基质组成影响了用于生物燃料应用的六醇,丁醇和乙醇生产.
科学领域:
- 生物技术和生物工程 生物技术和生物工程
- 可再生能源可再生能源是可再生能源.
- 微生物发酵 微生物发酵
背景情况:
- 可再生碳来源对于减少对化石燃料的依赖至关重要.
- 像Clostridia这样的细菌的合成气发酵提供了一条将CO或CO2 + H2转化为有价值的化学品和生物燃料的途径.
- 克洛斯特里碳酸是合成气发酵的关键微生物.
研究的目的:
- 为了评估Clostridium carboxidivorans在连续运行的水箱反应堆中的性能.
- 在不同稀释速率下,在pH 5.6下使用纯CO或合成合成气来评估酸和溶剂的生产.
- 了解基质组成和操作条件对发酵结果的影响.
主要方法:
- 在动反应堆中,对Clostridium carboxidivorans进行连续发酵.
- 在不同的稀释速率 (0.034-0.25小时-1) 中输入纯碳化合物或合成合成气.
- 监测细胞生长,酸和溶剂 (乙醇,butanol,hexanol) 的生产.
主要成果:
- 对于二氧化碳和合成气,发酵途径相似,在整个稀释速率下,在pH 5.6下持续增长和代谢物产生.
- 两种基板的稀释速率为0.25h-1时发生了冲洗.
- 乙醇是主要的溶剂,转向使用CO的butanol和使用合成气的hexanol.
- 用0.05小时-1.1的纯CO达到的最大细胞度 (0.5gDM/L)
- 最高的总溶剂生产率 (60毫克/升*小时) 在0.17小时−1.1时以合成合成气实现.
结论:
- 基质成分和操作条件显著影响合成气发酵效率.
- 克洛斯特里碳酸维在生物燃料和化学品生产的连续培养中表现出强大的性能.
- 优化合成气发酵对于可持续能源和化学制造至关重要.
关键词:
丁醇是一种醇.CSTR,CO,CO,CO,CO,CO. CSTR,CO,CO. CSTR,CO. CSTR,CO. CSTR,CO. CSTR,CO. CSTR,CO. CSTR,CO. CSTR,CO. CSTR,CO. CSTR,CO. CSTR,CO. CSTR,CO. CO. CSTR,CO. CSTR,CO. CSTR,CO. CO. CSTR,CO. CO. CSTR,CO. CO. CSTR,CO. CSTR,CO. CO. CSTR,CO. CSTR,CO. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR. CSTR.克洛斯特里 (Clostridium carboxidivorans) 是一种有机物.乙醇 乙醇是一种辛加斯天然气公司
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