生物过程优化,通过使用大米草的新分离物Meyerozyma caribbica CP02来增强利醇合成
Saumya Singh1,2, Shailendra Kumar Arya1, Meena Krishania3
1Department of Biotechnology, University Institute of Engineering and Technology, Panjab University, Chandigarh, India.
Biotechnology for biofuels and bioproducts
|February 24, 2024
概括
米草水解剂中的Meyerozyma caribbica CP02增强了从草水解剂中生成的西利的产生. 这种酵母分离物表现出强度和高发酵性,使其适合生物炼油厂,加工量最小.
科学领域:
- 生物技术是生物技术.
- 微生物发酵 微生物发酵
- 生物精炼是生物精炼的方法之一.
背景情况:
- 糖醇是一种有价值的糖醇,需求日益增长.
- 米是一种丰富的纤维素生物质资源.
- 有效的微生物转化纤维素衍生糖对于可持续生产至关重要.
研究的目的:
- 为了优化发酵参数,以提高Meyerozyma caribbica CP02.02的糖醇生产.
- 为了评估米水解盐的发酵性,用于生产利醇.
- 为了评估M. caribbica CP02在具有挑战性的条件下的强度.
主要方法:
- 一个变量一次 (OVAT) 优化发酵参数.
- 优化条件的统计验证.
- 使用商业化西洛斯和大米水解剂进行发酵研究.
- 在3L批量生物反应器中进行扩展.
主要成果:
- 优化条件包括32°C,pH3.5,200rpm动,1.5%的注射剂,以及80gL-1的初始氧化.
- 获得了0.77g−1 (商业用西洛) 和0.64g−1 (米西洛) 的氧化醇产量.
- 在3L生物反应器中,使用米水解和抑制剂,获得了0.63g-1的产量.
结论:
- 米草水解酸的Meyerozyma caribbica CP02显示出强大的发酵能力,用于生产利醇.
- 优化条件和分离物对抑制剂的耐受性有利于工业应用.
- 这种酵母有可能在生物炼油厂生产高产量利醇,减少预处理需求.
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