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Updated: Aug 2, 2026

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
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Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

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一种简化方法用于结构阐明,使用细胞内结晶的重组蛋白,InCellCryst

Robert Schönherr1, Juliane Boger1, J Mia Lahey-Rudolph1,2,3

  • 1Institute of Biochemistry, University of Lübeck, Lübeck, Germany.

Nature communications
|February 24, 2024
PubMed
概括

InCellCryst在昆虫细胞内产生均的蛋白质微晶,简化了结构生物学. 这种方法绕过了蛋白质净化,加速了X射线结晶学数据收集.

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相关概念视频

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...