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通过体外基于效应的测定在废水处理厂中的雌激素活性:洞察提取阶段的洞察

Marta Gea¹, Federica Spina², Roberta Revello¹

¹Department of Public Health and Pediatrics, University of Torino, Torino, Italy.

Journal of environmental management

|February 25, 2024

概括

污水处理厂的废水可以传播内分泌干扰物. 这项研究发现,提取方法和生物测试显著影响雌激素活性测量,强调需要标准化的水质监测来保护人类健康.

科学领域:

环境科学环境科学
毒理学毒理学毒理学
分析化学分析化学

背景情况:

污水处理厂 (WWTP) 的废水是水生环境中内分泌干扰化学物质 (EDC) 的重要来源.
基于效果的工具对于水质监测雌激素活性至关重要,但它们的结果可能受到各种因素的影响.
需要标准化方法来确保可靠地评估EDC所带来的环境风险.

研究的目的:

调查样本提取的不同静止阶段和不同的基于体外效应的试验如何影响WWTP废水中雌激素活性的测量.
为了比较两个提取静止阶段 (HLB和C18) 和两个生物试验 (哺乳动物雌激素相关NEWS试验[MELN]和酵母雌激素镜试验[YES]) 的性能.
确定最适合用于精确生物监测和水中的雌激素化合物的环境风险评估的方法.

主要方法:

来自意大利北部6个水上污水处理厂的季节性污水采样 (n=72个样本).
使用两个不同的静止阶段提取雌激素化合物:水友性-脂友性平衡 (HLB) 和八基 (C18).
使用两种以体外效应为基础的测试来测试提取的样本:一种哺乳动物细胞上的基因记者光酶测试 (MELN) 和酵母雌激素查测试 (YES).

主要成果:

雌激素活性测量在HLB和C18提取物之间有显著差异,表明提取阶段影响生物监测数据.
MELN试验始终显示出比YES试验更高的雌激素活性,这表明细胞膜透性和代谢激活的差异影响了结果.
MELN数据显示了废水中雌激素活性带来的潜在环境风险,而YES数据表明没有威胁,强调了依赖测试的风险评估.

结论:

静止阶段和生物试验的选择极大地影响了对水样中的雌激素活性的评估.
C18吸附剂提取和MELN测定显示出更高的灵敏度来检测雌激素活性和评估环境风险.
提取和生物测试方法的标准化对于可靠的水质监测和有效保护人类和环境健康至关重要.

关键词:

生物监测生物监测内分泌干扰物干扰内分泌干扰物 Estradiol 的度相当于 Estradiol 的度.提取吸附剂是一种提取吸附剂.工业废水工业废水交易活化试验.

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