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在大肠杆菌上表达碳酸无水酶的表面表达,作为一种可持续的方法,用于酶性CO2捕获

Juned Ali¹, Shazia Faridi¹, Amuliya Kashyap²

¹Enzyme Technology Lab, Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, India.

Enzyme and microbial technology

|February 25, 2024

概括

这项研究通过使用AIDA-I自传媒器将其显示在大肠杆菌上来设计Bacillus subtilis碳酸酶 (BhCA) 来捕获二氧化碳. 表面显示的BhCA显示了增强的稳定性和可重复使用性,提供了具有成本效益的碳捕获解决方案.

科学领域:

生物技术和生物催化剂
环境工程环境工程
分子生物学分子生物学

背景情况:

碳酸 anhydrase (CA) 是二氧化碳封存的关键催化剂,但在可溶性形式下,其稳定性较差,生产成本较高.
工业二氧化碳捕获需要强大且具有成本效益的生物催化剂来减轻对环境的影响.

研究的目的:

为了设计一个经济高效和稳定的二氧化碳脱水酶 (BhCA) 通过表面显示在E. coli上捕获二氧化碳.
在工业应用中克服可溶性CA酶的局限性.

主要方法:

细菌哈洛杜兰斯碳酸酶 (BhCA) 基因与AIDA-I自传媒体的基因融合,用于E. coli的表面显示.
通过使用FITC标记的抗体,FACS,细胞分离和zymography验证表面显示.
全细胞生物催化剂的稳定性,可重复使用性和耐性的生物化学表征.

主要成果:

实现了BhCA的有效表面显示 (595±60U/克干细胞重量).
增强的热稳定性 (T1/2在50°C:90分钟,60°C:36分钟,80°C:18分钟) 和显著的可重复使用性 (在15个循环后100%的活性).
表面显示的BhCA表现出与其可溶性对应物相比,具有可比的稳定性.

结论:

使用AIDA-I自动传送器对BhCA的表面显示是一种可行的战略,用于成本有效的碳捕获.
设计的全细胞生物催化剂显示出卓越的稳定性和可重复使用性,解决了可溶性CA的局限性.
这代表了CA表面显示使用AIDA-1自动传送器用于增强的二氧化碳捕获技术的第一份报告.

关键词:

细菌哈洛杜兰斯 (Bacillus halodurans) 是一种植物.碳酸无水酶是一种碳酸无水酶.支付-I 的时间.表面显示显示器的表面显示器全细胞生物催化剂全细胞生物催化剂

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