相关实验视频
Updated: Jul 2, 2025

08:38
Targeted DNA Methylation Analysis by Next-generation Sequencing
Published on: February 24, 2015
37.1K
在葡萄中进行全基因组DNA甲基化动态和RNA-seq分析 (参见葡萄中的DNA甲基化动态和RNA-seq分析) "卡伯纳法兰克") 的皮肤颜色
Ao Li1, Fengxia Wang1, Tingting Ding1
1Shandong Academy of Grape, Jinan 250100, China.
Genomics
|February 25, 2024
概括
葡萄皮DNA甲基化模式在颜色变化期间被映射出来. 发现有差异的甲基化区域,特别是在CHH环境中,与黄类生物合成和葡萄颜色有关.
科学领域:
- 植物生物学 植物生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
背景情况:
- 葡萄皮的颜色是一个复杂的过程,受到各种因素的影响.
- 了解表观遗传机制,如DNA甲基化,对于葡萄的发展至关重要.
研究的目的:
- 为了生成葡萄皮的全基因组DNA甲基化地图 (cv. "卡伯内法兰克") 的产品.
- 在葡萄皮色化过程中对DNA甲基组进行表征.
- 为了检查DNA甲基化在这个过程中的功能意义.
主要方法:
- 在葡萄皮组织上进行了全基因组DNA甲基化映射.
- 在三个关键发育阶段采集样本:早期胀,晚期胀和真实胀.
- 鉴定和分析了差异甲基化区域 (DMR).
主要成果:
- 在可转移元素区域,DNA甲基化水平高于基因区域.
- 在基因区域内,CG和CHG甲基化水平高于CHH.
- 在染色过程中,DMRs主要发生在CHH环境中,在核酸结合和黄酸生物合成途径中富含基因.
结论:
- 基因甲基化,特别是在CHH的背景下,在葡萄皮肤颜色中起着重要作用.
- 鉴定到的DMR和相关基因为我们提供了关于弗拉类生物合成的表观遗传调节的见解.
- 这项研究为进一步研究葡萄的发育机制奠定了基础.
相关概念视频
Position-effect Variegation
6.3K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
6.3K
Epigenetic Regulation
3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
3.0K

