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探索衰老对植入和早期胚胎发育的影响,使用全能细胞衍生的布拉斯托伊德

Yuxin Luo¹, Chenrui An², Ke Zhong²

¹State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China; National Clinical Research Center for Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China; Key Laboratory of Assisted Reproduction, Ministry of Education, Peking University Third Hospital, Beijing 100191, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Peking University Third Hospital, Beijing 100191, China.

Journal of advanced research

|February 25, 2024

概括

衰老会损害胚胎植入,这是降低孕育率的关键因素. 全能芽质体样细胞芽质体 (TBL-芽质体) 为研究这些植入失败提供了一种新型模型.

科学领域:

生殖生物学生殖生物学
发育生物学是发展生物学.
细胞衰老细胞衰老

背景情况:

先进的孕产妇年龄与较低的植入率和怀孕成功有关.
与年龄相关的生育率下降背后的机制尚未完全理解.
现有的植入研究模型是有限的.

研究的目的:

研究衰老对胚胎植入能力的影响.
开发和利用一种新的布拉斯泰德模型来研究植入.
为了阐明植入障碍的潜在治疗点.

主要方法:

使用全能芽质体样细胞 (TBLCs) 构建TBL-blastoids.
通过氧化应激诱导的TBLCs生成与衰老相关的胚胎模型.
在小鼠模型中进行了体外和体外植入试验.
转录基因分析TBL-blastoids和人类的决定性.

主要成果:

TBL-blastoids重复了胚胎细胞的发育和植入潜力.
在TBL-blastoids中的衰老显著损害了植入,反映了与年龄相关的生育问题.
转录组分析确定了与生育疾病和妊娠并发症相关的差异表达基因.
在TBL-blastoids和deciduae中常见的差异表达基因表明潜在的治疗点.

结论:

TBL-blastoids作为研究植入和早期植入后发育的强大模型.
这个模型提供了有关与晚年孕产妇年龄相关的怀孕障碍的见解.
鉴定的基因可能为改善植入率提供新的治疗策略.

关键词:

胚胎细胞囊 (Blastocyst) 是一种细胞囊.布拉斯托伊德 (Blastoids) 是一种类型.植入植入植入植入植入植入植入植入衰老是一种衰老.在Totipotent中,它是非常强大的.

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