一种脊椎动物Vangl2转化变体,需要平面细胞极性
Alexandra Walton1, Virginie Thomé2, Diego Revinski2
1Aix Marseille Univ, CNRS, INSERM, Institut Paoli-Calmettes, CRCM, Equipe labellisée Ligue 'Cell Polarity, Cell Signaling And Cancer', Marseille, France.
The Journal of biological chemistry
|February 25, 2024
概括
一种新发现的Vangl2-Long蛋白质异型对脊椎动物的胚胎发育和平面细胞极性 (PCP) 至关重要. 这种异构体在胚胎延伸和神经管闭合中起着至关重要的作用,维持细胞极性.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 平面细胞极性 (PCP) 是甲基动物的一个基本的发育过程,对胚胎发生和极化结构的形成至关重要.
- 编码跨膜蛋白的Vangl基因家族对于PCP信号传递至关重要,Vangl2在各种物种的胚胎发育中发挥着关键作用.
研究的目的:
- 在分子和功能上表征一种新型的Vangl2异型,称为Vangl2-Long,通过另一种翻译启动站点识别.
- 调查Vangl2-Long在胚胎发育中的作用,特别是在Xenopus中,以及其对平面细胞极性的贡献.
主要方法:
- 识别和表征Vangl2-Long异型,包括其独特的N端延伸.
- 在Xenopus胚胎中利用morpholino oligonucleotides对Vangl2-Long的特定淘汰.
- 分析Vangl2-Long在Vangl1和Vangl2.2的多重复合物形成中的作用.
- 评估Vangl2-Long对胚胎延伸,神经管闭合和PCP分子偏振分布的影响.
主要成果:
- Vangl2-Long具有在脊椎动物Vangl2中保留的N端延伸,但在Vangl1和无脊椎动物中不存在.
- Vangl2-Long是功能性的,对于Xenopus胚胎延伸和神经管闭合至关重要.
- 对于Prickle2 (Pk2) 和Dishevelled1 (Dvl1) 的极化分布以及中心点旋转极性,需要正确表达Vangl2和Vangl2-Long.
结论:
- Vangl2-Long是一种特定于脊椎动物的Vangl2异型,起源于一个替代的翻译起点.
- 这种异型是PCP通路的功能组成部分,对于关键的胚胎发育事件是不可或缺的.
- Vangl2-Long对血膜中的Vangl2蛋白池有显著的贡献,从而保持脊椎动物组织中的平面细胞极性.
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