排卵和授精之间的时间决定了受精的老鼠卵细胞的质量
Naomi Nakagata1, Satohiro Nakao2, Nobuyuki Mikoda1,3
1Division of Reproductive Biotechnology and Innovation, Center for Animal Resources and Development (CARD), Institute of Resource Development and Analysis, Kumamoto University, Kumamoto 860-0811, Japan.
The Journal of reproduction and development
|February 25, 2024
概括
确定大鼠卵细胞采集的最佳时间对于体外受精 (IVF) 成功至关重要. 这项研究发现,大鼠卵细胞在采集后4小时内保持高发育能力用于试管婴儿.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
背景情况:
- 转基因大鼠是人类疾病研究的重要模型.
- 有效的冷保存和体外受精 (IVF) 系统对于生产和保存这些有价值的模型至关重要.
- 目前的试管婴儿协议要求固定时间表,限制了激素施用和卵细胞采集的灵活性.
研究的目的:
- 为了确定用于试管婴儿的老鼠卵细胞采集的最佳时间窗口.
- 评估各种化时间后排卵的老鼠卵子细胞的生育能力和发育潜力.
- 加强对转基因大鼠生产的试管婴儿计划的灵活性.
主要方法:
- 鼠卵细胞在上午9点收集,并化1-13小时.
- 在试管婴儿后,用冷解的老鼠精子评估了受精率.
- 评估了卵细胞发育能力,并监测了活幼的产量.
主要成果:
- 采集后7小时,受精率开始下降.
- 在卵细胞收集后10小时观察到异常受精.
- 虽然发育率在7小时后下降,但成功地从12小时前收集的卵细胞中获得了活幼.
结论:
- 产卵后的老鼠卵细胞在采集后4小时内保持高受精率和用于试管婴儿的发展潜力.
- 将窗口延长到收集后12小时是可能的,尽管成功率较低.
- 这些发现支持对转基因大鼠研究的试管婴儿计划更大的灵活性.
相关概念视频
Oogenesis
63.7K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
63.7K
In Vitro Fertilization
261
In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...
261


