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用人工神经网络,表征和抗瘤活动来优化Streptomyces albogriseolus黄金纳米颗粒生物合成的过程

Noura El-Ahmady El-Naggar¹, Asmaa A El-Sawah², Mohamed F Elmansy³

¹Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El- Arab City, Alexandria, 21934, Egypt. nouraalahmady@yahoo.com.

Scientific reports

|February 25, 2024

概括

这项研究提出了一种环保的方法,利用Streptomyces albogriseolus合成金纳米粒子 (GNP). 合成的GNP显示出有前途的抗癌活性,特别是与多克索鲁比结合时,这表明增强了癌细胞向.

科学领域:

纳米技术纳米技术
生物技术是生物技术.
材料科学材料科学材料科学

背景情况:

金纳米粒子 (GNP) 在各种生物医学应用中表现出显著的潜力,包括癌症治疗,药物输送和成像.
对于纳米粒子生物合成的可持续和环保方法的需求越来越大.
星菌 (Streptomyces albogriseolus) 为纳米粒子合成提供了一个潜在的生物来源.

研究的目的:

开发一种环保的金纳米颗粒生物合成方法,使用Streptomyces albogriseolus.的无细胞超体.
描述生物合成的GNP并优化其生产产量.
为了评估合成的GNP的体外抗癌活性,单独和与多克索鲁比结合.

主要方法:

使用Streptomyces albogriseolus的无细胞超体作为减少和稳定剂的GNP的生物合成.
使用UV-Vis光谱,传输电子显微镜 (TEM),泽塔电位,FTIR,X射线衍射 (XRD) 和选区电子衍射 (SAED) 进行GNP的表征.
使用中央复合设计 (CCD) 和人工神经网络 (ANN) 为产量预测优化GNP生物合成.

主要成果:

大小从5.42到13.34纳米,负电荷表面 (-24.8mV) 的球形GNP已成功生物合成.
FTIR,XRD和SAED证实了功能组的存在和GNP的晶体结构.
确定了最大GNP产量的最佳条件 (778.74μg/mL),ANN与CCD相比显示出更高的预测准确性.
生物合成的GNP在体外表现出对HeP-G2细胞系的抗癌活性,与多克索鲁比 (IC50为3.52μg/mL) 结合时有效性得到增强.

结论:

星菌 (Streptomyces albogriseolus supernatant) 为GNP生物合成提供了一个有效和环保的平台.
生物合成的GNP具有有利的物理化学特性和显著的抗癌潜力.
用多克索鲁比辛和生物合成的GNP进行组合疗法为增强癌症治疗提供了一个有希望的策略.

关键词:

人工神经网络的人工神经网络生物合成生物合成中心复合设计设计标志性描述标志性描述国家统计计划 (GNP) 是一个国家统计计划.人类癌症细胞系HeP-G2 过程优化过程优化 Streptomyces albogriseolus 这种菌株的属性是什么?

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