细菌氨酸生物合成的植物学引导性表征,由库宾/甲基tRNA合成酶类酶介导
Kenichi Matsuda1, Yuto Nakahara1, Atina Rizkiya Choirunnisa1
1Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita-ku, Sapporo, 060-0812, Japan.
Chembiochem : a European journal of chemical biology
|February 26, 2024
概括
研究人员探索了酶形成-键的新方法,发现了新的素产品. 这扩大了我们对天然产品生物合成和酶能力的理解.
科学领域:
- 生物化学 生物化学
- 酶学 是一种酶学.
- 自然产品生物合成 自然产品生物合成
背景情况:
- 类似于cupin/methionyl-tRNA合成酶 (MetRS) 的酶在天然产品合成中形成关键的- (N-N) 键.
- 由这些酶生成的氨酸是关键的生物合成中间体,但它们的完整结构多样性尚未得到充分理解.
研究的目的:
- 探索由cupin/MetRS类酶产生的氨酸产品的结构多样性.
- 确定新的N-N键形成途径和酶功能.
主要方法:
- 基因组导向的基因组挖掘氨酸生物合成途径.
- 亚黄素依赖的Nω-氧化单氧酶 (NMO) 和cupin/MetRS类酶的生物化学表征.
主要成果:
- 在NMO和cupin/MetRS类酶家族中确定了尚未探索的序列空间.
- 证明了NMO产生新型Nω-氧胺作为前体的能力.
- 发现了五种新的氨酸产品,包括含有非氨基酸构成块的产品,如1,3-二氨基和氨酸.
结论:
- 实质性地扩大了已知的N-N结合形成途径,由库宾/MetRS类酶介导.
- 揭示了产生各种水结构的新型酶能力.
- 开辟了天然产品发现和合成生物学的新途径.
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