在RiPP生物合成过程中参与领头去除的蛋白质
Sara M Eslami1, Wilfred A van der Donk1,2
1Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
ACS bio & med chem Au
|February 26, 2024
概括
本综述探讨了从核糖体合成和翻译后修饰 (RiPPs) 中去除领导的细菌蛋白酶. 这些蛋白酶对于产生生物活性RiPPs至关重要,并表现出不同的裂变策略和基质特异性.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 自然产品生物合成 自然产品生物合成
背景情况:
- 核糖体合成和翻译后改性 (RiPPs) 是一类具有显著生物活性的多样化天然产品.
- 它们的生物合成途径是可移植的,这使得它们成为异质表达的有吸引力的目标.
- 领导通常在异质表达过程中被保留,以防止宿主毒性和促进净化.
研究的目的:
- 审查细菌蛋白质酶的表征方面的进展,参与RiPP领导去除.
- 要突出这些蛋白酶作为依赖序列的酶的实用性.
- 讨论用于领导裂的各种策略和机制.
主要方法:
- 对细菌RiPP生物合成酶和相关蛋白酶的研究进行文献综述.
- 从细菌RiPP系统中分析特征化的相关蛋白酶.
- 检查报告的领导去除策略,包括蛋白酶类型,局部化和基质特异性.
主要成果:
- 细菌RiPP相关蛋白酶在它们的分裂机制中表现出了显著的多样性,从单一蛋白酶到多步骤过程.
- 蛋白质分解裂变可以发生在各种细胞位置:细胞质上,分泌过程中或细胞外.
- 这些蛋白酶的基质识别从高度序列特异性到与领导者和/或核心的非序列特异性相互作用而有所不同.
结论:
- 细菌RiPP相关蛋白酶是生产成熟RiPP的多功能工具.
- 了解它们的多样化机制可以增强RiPP生物合成的工程.
- 这些蛋白酶在生物技术中作为依赖序列的酶提供了有价值的应用.
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