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Updated: Jun 27, 2026

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

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优化核定位信号组合 改善CRISPR-Cas12a编辑速率 在人类初级细胞中

Kevin Luk1, Pengpeng Liu1, Jing Zeng2,3,4

  • 1Department of Molecular, Cell and Cancer Biology, Li Weibo Institute for Rare Diseases Research, University of Massachusetts Chan Medical School, Worcester, MA, USA.

GEN biotechnology
|February 26, 2024
PubMed
概括

研究人员优化了CRISPR-Cas12a (一种基因组编辑工具),以增强其在人类细胞中的活性. 这种改进的Cas12a系统在原始细胞中显示出强大的编辑能力,而不会增加非目标突变,为基因治疗提供了一个有前途的工具.

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相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...