水晶紫色染料的生物降解和脱色通过与真菌和细菌共同培养
Yongqiang Tian1, Kangli Wu1, Shenghong Lin1
1School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China.
ACS omega
|February 26, 2024
概括
菌和细菌共同培养有效降解了水晶紫色染料,表现优于单个菌株. 这种微生物处理可以对染料进行排毒,为生物修复提供了有前途的应用.
科学领域:
- 环境微生物学环境微生物学
- 生物技术是生物技术.
- 生物修复是一种生物修复.
背景情况:
- 微生物染料降解对环境安全至关重要.
- 水晶紫 (CV) 是一种广泛使用的染料,具有潜在的生态影响.
- 了解染料脱色的微生物联盟是必不可少的.
研究的目的:
- 研究真菌 (Aspergillus fumigatus A-3) 和细菌 (Pseudomonas fluorescens L-6) 共同培养在降解水晶紫 (CV) 染料中的有效性.
- 为了比较共同种植和单一种植的脱色效率.
- 为了确定参与CV生物降解的酶和降解产品.
主要方法:
- 鉴定出真菌和细菌菌株为Aspergillus fumigatus A-3和Pseudomonas fluorescens L-6.这些菌株分别是真菌菌和细菌菌株.
- 对CV染料脱色进行了共同培养和单一培养实验.
- 进行了酶分析,以确定关键酶 (Lac,MnP,Lip,NADH-DCIP减少酶).
- 使用光谱 (UV-Vis,FT-IR) 和色谱 (GC-MS) 技术来分析降解产品.
- 植物毒性测试评估了CV及其降解产品的毒性.
主要成果:
- 同种植物表现出较高的CV脱色率相比单种植物,无论是Aspergillus fumigatus A-3或Pseudomonas fluorescens L-6.
- 包括Lac,MnP,Lip和NADH-DCIP还原酶在内的酶参与了生物降解过程.
- 气色谱-质谱检测确定了降解产物,如4- ((dimethylamino) ,3-dimethylaminophenol,醇和甲.
- 与母CV染料相比,降解产品的植物毒性降低,这表明排毒.
结论:
- 菌-细菌共同培养对晶体紫色染料脱色非常有效.
- 已识别的酶和降解途径有助于CV生物降解.
- 共同种植的CV排毒突出了它们对环境生物修复应用的潜力.
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