相关实验视频

Updated: Jul 2, 2025

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Published on: September 14, 2014

在活体中,CD4的HIV-1 Env-binding域的亲和力成熟

Andi Pan^1,2,3,4,5, Charles C Bailey², Tianling Ou^2,3,4

¹Skaggs Graduate School, Scripps Research, La Jolla, CA 92037, USA.

Research square

|February 26, 2024

概括

工程B细胞表达人类蛋白质生物体在体内表现出增强的亲和力成熟. 这种方法显著改善了HIV-1生物药物的中和效能,而不会影响药理动力学.

科学领域:

生物技术是生物技术.
免疫学免疫学免疫学
蛋白质工程是指蛋白质的工程.

背景情况:

人类蛋白质经常被改造成生物制剂,但体外方法无法优化与疗效相关的特征,如蛋白酶敏感性.
目前的蛋白质工程方法可能无法完全解决体内疗效方面的挑战.

结论:

非抗体蛋白生物制剂的体内亲和力成熟是可行的和有效的.
这一战略可以指导开发更强效的基于蛋白质的疗法.
直接在B细胞受体框架内工程生物提供了一条新的治疗开发途径.

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Assessment of Immunologically Relevant Dynamic Tertiary Structural Features of the HIV-1 V3 Loop Crown R2 Sequence by ab initio Folding

Assessment of Immunologically Relevant Dynamic Tertiary Structural Features of the HIV-1 V3 Loop Crown R2 Sequence by ab initio Folding

Published on: September 15, 2010

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Published on: March 8, 2012

相关概念视频

T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...