在T细胞激活后,需要NF-κB信号来维持X染色体无活化
bioRxiv : the preprint server for biology
|February 26, 2024
概括
女T细胞使用NF-κB信号来维持X染色体不活化 (XCI). T细胞激活触发XistRNA积累和非活跃X染色体 (Xi) 上的表观遗传变化,影响基因表达.
科学领域:
- 遗传学 遗传学是一种遗传学.
- 免疫学 免疫学 免疫学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- X染色体不活化 (XCI) 平衡了两性之间的基因剂量.
- 在未刺激的T细胞中,不活跃的X染色体 (Xi) 缺乏特定的RNA和表观遗传标记.
- 刺激T细胞导致XI上这些标记的丰富.
研究的目的:
- 研究T细胞刺激后XI的等位基因特异性基因表达和表观基因特征.
- 确定调节XistRNA局部化到Xi的T细胞信号通路.
主要方法:
- 对基因特异性基因表达和Xi上的表观基因组概况的分析.
- 在T细胞刺激后检查XistRNA局部化.
- 使用抑制剂和遗传模型,研究NF-κB信号通路在XCI维持中的作用.
主要成果:
- 未刺激的T细胞显示剂量补偿和H3K27me3在Xi上的丰富,但没有H2AK119-ubiquitin (Ub).
- 刺激T细胞导致H2AK119-Ub和H3K27me3.3的Xi积累.
- T细胞受体 (TCR) 参与和下游NF-κB信号传递对于XistRNA定位到Xi至关重要.
结论:
- 在雌性T细胞中,NF-κB信号传递对XistRNA积累和XCI维持至关重要.
- 破坏NF-κB信号传递会改变X链基因表达.
- 这项研究揭示了NF-κB信号传递和T细胞中的XCI之间的新联系.
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