德洛索菲拉基因组甲基转移酶SET1协调多个信号通路,调节男性生殖系干细胞的维持和分化
Velinda Vidaurre1, Annabelle Song1, Taibo Li1
1Department of Biology, The Johns Hopkins University, Baltimore, Maryland, United States of America.
bioRxiv : the preprint server for biology
|February 26, 2024
概括
组织素甲基转移酶Set1调节了Drosophila的干细胞平衡. 抑制Set1导致生殖细胞丧失,然后过度繁殖,影响细胞并引起癌症治疗的担忧.
科学领域:
- 发展生物学 发展生物学
- 干细胞生物学 干细胞生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 成人干细胞通过平衡的增殖和分化来维持组织平衡.
- 基因组甲基转移酶Set1 (H3K4me3) 在调节基因表达和细胞过程中起着至关重要的作用.
研究的目的:
- 研究Set1在调节Drosophila早期男性生殖细胞增殖和分化中的作用.
- 阐明Set1控制干细胞行为和利基互动的分子机制.
主要方法:
- 在Drosophila. 的 *set1* 基因的生殖系特异性淘汰.
- 对生殖细胞增殖,分化和利基结构的分析.
- RNA测序 (RNA-seq) 用于识别失调的信号通路.
- 基因相互作用测定与JAK-STAT和BMP通路组件.
主要成果:
- *set1* knockdown导致了暂时渐进的缺陷,包括生殖细胞的丧失和随后的过度繁殖.
- 生殖系缺陷影响了利基架构和囊干细胞系的非细胞自主性.
- Set1的甲基转移酶活性对于拯救 * set1 * 敲击表型至关重要.
- RNA-seq发现了JAK-STAT (*stat92E*) 和BMP (*mad*) 途径基因的上调.
- 基因相互作用证实了JAK-STAT和BMP通路在Set1-介导调节中的参与.
结论:
- Set1对于保持Drosophila雄性生殖干细胞的增殖和分化之间的平衡至关重要.
- Set1通过对的非细胞自主作用和与关键信号通路的相互作用来调节生殖线的发育.
- 观察到的表型和途径失调表明,在癌症治疗中使用组氨基甲基转移酶抑制剂可能存在问题.
相关概念视频
01:14Maintenance of the ES Cell State
2.2K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.2K
02:45The Ratio of X Chromosome to Autosomes
8.5K
In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
8.5K
03:17Inheritance of Chromatin Structures
6.2K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.2K
02:50Dosage Compensation
6.2K
In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
6.2K