来自尼泊尔的急性脑炎患者中检测到Gemykibivirus
Eans Tara Tuladhar1, Smita Shrestha1, Susan Vernon2
1Tribhuvan University Central Department of Biotechnology, Kathmandu, Nepal.
medRxiv : the preprint server for health sciences
|February 26, 2024
概括
在尼泊尔患有急性脑炎综合征 (AES) 的儿童中发现了一种新型的gemykibivirus. 这一发现为无法解释的脑炎病例提供了潜在的原因,改善了诊断和治疗策略.
科学领域:
- 病毒学 病毒学
- 神经学 神经学
- 传染性疾病 传染性疾病
背景情况:
- 急性脑炎综合征 (AES) 是一个重大的全球健康挑战,导致大量的发病率和死亡率.
- 在尼泊尔,日本脑炎病毒 (JEV) 仅在5-20%的AES病例中被发现,大约75%的病例的病因不明.
- 尼泊尔大多数AES病例的致病因子仍然在很大程度上未被确定.
研究的目的:
- 确定与尼泊尔无法解释的急性脑炎综合征 (AES) 相关的新型病毒病原体.
- 在患者样本中描述新发现病毒的遗传构成和流行率.
- 建立诊断工具,用于在临床标本中检测识别的病毒病原体.
主要方法:
- 甲基因组下一代测序 (mNGS) 用于分析儿科AES病例中的脑脊液 (CSF).
- 生物信息分析用于从测序数据中组装和识别病毒基因组.
- 开发了实时PCR测定,并使用它来选更大的AES患者样本队列 (CSF和血清).
- 在阳性样本上进行了全基因组测序,以确认和进一步描述已识别的病毒.
主要成果:
- 一种新型的gemykibivirus,一种单链圆形DNA病毒,在患有AES的儿童的脑脊液中被发现.
- 鉴定出gemykibivirus的完整基因组被测序,显示高核酸相同性 (98.69%) 与人类相关的gemykibivirus 2.
- 通过PCR对337个脊髓和164个血清样本进行查,发现11个脊髓和1个血清样本对gemykibivirus呈阳性.
- 测序了7个阳性样本的完整基因组,证实了病毒的存在和遗传多样性.
结论:
- 这项研究确定了一种gemykibivirus作为尼泊尔脑炎的潜在致病原体.
- 这些发现有助于了解该地区无法解释的AES病例的病因.
- 开发的PCR分析为未来的监测和临床管理提供了宝贵的诊断工具.
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