使用负感RNA病毒核蛋白的结构预测来评估进化关系
Kimberly R Sabsay1,2, Aartjan J W Te Velthuis1
1Lewis Thomas Laboratory, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, United States.
bioRxiv : the preprint server for biology
|February 26, 2024
概括
使用核蛋白 (NP) 结构研究了负感RNA病毒 (NSV) 的演变. NP结构揭示了与RNA指导RNA聚合酶 (RdRp) 数据一致的进化关系,有助于病毒分类.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 负感官RNA病毒 (NSV) 是人类显著的病原体.
- 目前的NSV分类依赖于RNA指导的RNA聚合酶 (RdRp) 域序列.
- 与RdRp一起核蛋白 (NP) 的进化轨迹尚未完全理解.
研究的目的:
- 调查核蛋白 (NP) 结构是否可用于推断负感RNA病毒 (NSV) 内的进化关系.
- 为了确定NP的演变是否与RdRp域的演变平行.
- 评估NP序列对病毒分类的有用性,特别是当缺少RdRp数据时.
主要方法:
- 使用实验获得的和AlphaFold 2.0预测的NP结构.
- 执行模拟NP结构的灵活结构对齐.
- 基于NP的族系学群与现有的基于RdRp的分类进行了比较.
主要成果:
- NSV NP 的结构同质性揭示了与基于 RdRp 的聚类相一致的基因组群.
- 缺乏RdRp序列的病毒使用NP序列成功被分配到基因组群中.
- 基于RdRp和NP的分析都建议重新分类细分的Naedrevirales.
结论:
- NSV RdRp和NP基因在很大程度上沿着类似的轨迹进化.
- NP结构为推断NSV中的进化关系提供了有价值的工具.
- 短遗传序列,特别是NP,可以增强对病毒发现和分类的元基因组分析的价值.
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