生物原银纳米颗粒在浮游生物和生物膜相关的Corynebacterium伪结核病中的活性
Laerte Marlon Santos1, Daniela Méria Rodrigues1, Bianca Vilas Boas Alves1
1Instituto de Ciencias da Saude, Universidade Federal da Bahia, Salvador, Bahia, Brazil.
PeerJ
|February 26, 2024
概括
银纳米颗粒 (AgNPs) 显示出在治疗病例性淋巴腺炎 (CL) 中具有显著的潜力,通过有效抑制Corynebacterium伪结核病的生长和破坏其保护生物膜,为传统抗生素提供了有希望的替代品.
科学领域:
- 兽医微生物学 兽医微生物学
- 纳米技术纳米技术
- 抗菌剂 抗菌剂 抗菌剂
背景情况:
- 科里尼细菌伪结核病在小反动物中引起状淋巴腺炎 (CL),这种疾病不耐抗生素治疗.
- 生物膜形成是C. pseudotuberculosis的关键毒性因素,有助于抗菌素耐药性.
- 银纳米粒子 (AgNPs) 呈现出具有低抗性潜力的广谱抗菌和抗菌膜活性.
研究的目的:
- 评估银纳米颗粒 (AgNPs) 对Corynebacterium pseudotuberculosis的抗微生物和抗菌膜疗效.
- 为了确定AgNP的最小抑制度 (MIC) 和最小杀菌度 (MBC).
- 评估AgNP对C. pseudotuberculosis生物膜形成和结构的影响.
主要方法:
- 对抗C. pseudotuberculosis的AgNPs的MIC和MBC值的确定.
- 使用干扰值评估AgNP抗菌膜活性.
- 通过扫描电子显微镜 (SEM) 可视化AgNP对生物膜结构的影响.
主要成果:
- AgNPs完全抑制了C.伪结核病的生长,并在0.08至0.312毫克/毫升的度下使细菌失活.
- AgNPs表现出显著的抗生物膜活性,在4mg/ml的剂量下降了80%以上的生物膜形成.
- 在SEM中,AgNPs对合并的C. pseudotuberculosis生物膜进行了实质性破坏.
结论:
- 银纳米颗粒有效地抑制了Corynebacterium伪结核病的生长.
- AgNPs显著减少生物膜的形成,并破坏现有的生物膜,这是C. pseudotuberculosis的关键毒性因素.
- 这些发现支持AgNPs作为治疗状淋巴腺炎的新型治疗剂的潜力.
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