隔离特定于引起牛乳炎的细菌菌的细菌菌体,并对它们在巴氏消毒牛奶中的溶解活性进行表征
Napakhwan Imklin1, Patinya Patikae2,3, Peekarn Poomirut2
1Department of Biotechnology, Faculty of Engineering and Industrial Technology, Silpakorn University, Nakhon Pathom, Thailand.
Veterinary world
|February 26, 2024
概括
菌体治疗在治疗由抗生素耐药细菌引起的牛乳炎方面表现有前途. 菌体尾酒有效降低了牛奶中的细菌数量,尽管在体内应用方面需要进一步的研究.
科学领域:
- 兽医微生物学 兽医微生物学
- 细菌生物学的生物学
- 乳制品科学 乳制品科学
背景情况:
- 牛乳炎对乳制品生产构成重大威胁,通常是由传染性和大肠杆菌病原体引起的.
- 抗生素耐药细菌的增加需要替代性治疗乳腺炎.
- 菌体治疗为控制抗生素耐药性乳腺炎病原体提供了一个可行的替代方案.
研究的目的:
- 为了隔离和描述向传染性和大肠杆菌性乳腺炎病原体的菌体.
- 评估隔离的菌体在降低巴氏消毒牛奶中的细菌负载中的有效性.
- 评估菌体尾酒在增强治疗效果方面的潜力.
主要方法:
- 从原牛奶和废水中分离了四种特定的细菌菌体 (vB_Sau-RP15,vB_Eco-RN12i1,vB_Kpn-RN14i1,vB_Ssc-RN20i3)
- 确定每个孤立的菌体的宿主范围和涂层效率 (EOP).
- 用单个菌体和菌体尾酒在不同感染次数 (MOI) 的情况下,对消毒奶中的菌体溶解活性进行评估.
主要成果:
- 所有孤立的菌体都对各自的细菌宿主表现出高度的特异性.
- 单个和尾菌体治疗在2小时内显著降低了增巴氏化牛奶中的细菌数量.
- 与单个菌体治疗相比,菌体尾酒显示出更高的疗效,尽管观察到归因于菌体不敏感突变的细菌再生.
结论:
- 菌体的应用是治疗牛乳腺炎的一个有希望的策略.
- 菌体尾酒可以提高在巴氏消毒牛奶中治疗乳腺炎的疗效.
- 对于乳牛的有效生物控制,对菌体内生和外生应用的菌体特性进行进一步的研究至关重要.
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