从子便中分离出来的乳酸细菌的酶特性
Dini Dwi Ludfiani1, Widya Asmara2, Forita Dyah Arianti1
1Research Center for Sustainable Production Systems and Life Cycle Assessment, National Research and Innovation Agency (BRIN), Tangerang Selatan, Indonesia.
Veterinary world
|February 26, 2024
概括
这项研究描述了子便中的乳酸细菌 (LAB) 酶,确定了两种优异菌株,Lactococcus lactis亚种. 对于潜在的发酵应用而言,乳酸K5和乳酸细菌Brevis M4A.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 酶学 是一种酶学.
背景情况:
- 印度尼西亚每年都会产生大量的纤维素生物质废物.
- 这些废物的发酵可以产生有价值的料和生物燃料.
- 乳酸细菌 (LAB) 是发酵的关键微生物,潜在的来源包括子便.
研究的目的:
- 从子便中分离和描述LAB酶.
- 为了确定LAB具有优越的发酵特性,用于生物质利用.
主要方法:
- 使用API ZYM套件从子便中获得11个LAB培养物的酶特征.
- 细菌细胞悬浮的化和观察试剂添加后的颜色变化.
主要成果:
- 在所有LAB中观察到中度的雌激酶活性.
- 在七个LAB中强烈的酸酶和氨基酸酶活性.
- 在8个LAB中发现了甘氨基酸酶 (GH) 活性,在Lactococcus lactis亚种中具有强烈活性. 乳糖K5和乳杆菌BrevisM4A. 和乳糖K5和乳酸菌BrevisM4A. 和乳糖K5和乳酸菌BrevisM4A.
结论:
- 有两个LAB菌株,L. lactis亚种. 乳糖K5和L. brevis M4A,表现出优越的酶特性.
- 这些菌株显示出发酵,基纤维素水解以及作为料添加剂的高潜力.
- 建议进行进一步的研究,以确定分子特征和特定活性.
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