相关实验视频

Updated: Jul 2, 2025

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

Published on: October 17, 2018

嵌入树脂的定量评估用于体积电子显微镜

Lennart Tegethoff¹, Kevin L Briggman¹

¹Max Planck Institute for Neurobiology of Behavior - caesar, Bonn, Germany.

Frontiers in neuroscience

|February 26, 2024

概括

实现一致的串行段需要优化环氧树脂嵌入. 本研究介绍了测量树脂固化均性和切削力的方法,以确保电子显微镜更好的断面厚度可重复性.

科学领域:

材料科学材料科学材料科学
显微镜技术显微镜技术
生物技术是生物技术.

背景情况:

在体积电子显微镜中,对大组织样本进行一致的连续切割至关重要.
不均的环氧树脂固化可以损害切片质量和均性.
目前的方法缺乏树脂固化及其对切割的影响的可靠量化.

研究的目的:

开发和验证用于量化环氧树脂固化不均性的方法.
评估树脂特性和嵌入技术对切割重复性的影响.
为优化树脂配方和嵌入协议提供一个框架,以提高切片质量.

主要方法:

使用块硬度测量量树脂固化不均性的量化.
用力传感器测量超薄切割过程中的横切割和切割力.
在环氧嵌入之前,对不规则形状的组织样本进行凝封装的探索.

主要成果:

确定导致树脂固化不均的条件.
建立了一种监测各种环氧树脂块硬度的程序.
证明选和优化树脂配方可以提高切片厚度的重复性.
展示了凝封装作为一种改善不规则样本截面均性的方法.

结论:

块硬度测量是一种有效的方法来量化树脂固化不均.
优化环氧树脂配方和嵌入程序对于一致的串行切割至关重要.
凝封装提供了一种可行的策略,用于提高具有挑战性的样本几何形状的切片质量.

关键词:

切割的力量,切割的力量.环氧树脂是一种环氧树脂.硬度硬度硬度硬度组织嵌入嵌入.卷电子显微镜 (vEM) 的使用

更多相关视频

High-resolution Episcopic Microscopy HREM - Simple and Robust Protocols for Processing and Visualizing Organic Materials

High-resolution Episcopic Microscopy HREM - Simple and Robust Protocols for Processing and Visualizing Organic Materials

Published on: July 7, 2017

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

相关概念视频

Preparation of Samples for Electron Microscopy

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...