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大麻二醇通过调节氧化应激来保护C2C12神经管免受西斯普拉丁诱导的缩

Olivier Le Bacquer¹, Phelipe Sanchez¹, Véronique Patrac¹

¹Unité de Nutrition Humaine (UNH), Université Clermont Auvergne, INRAE, CRNH Auvergne, Clermont-Ferrand, France.

American journal of physiology. Cell physiology

|February 26, 2024

概括

在细胞模型中,大麻素 (CBD) 抵消因西斯普拉丁化疗引起的肌肉损失. 这项研究表明,CBD可能有助于保持肌肉质量,并在癌症治疗期间改善患者的生活质量.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
药理学药理学是指药理学的学科.

背景情况:

癌症和化疗,像西斯普拉丁一样,诱导肌肉消耗 (缓解),对患者的结果产生负面影响.
了解化疗引起的肌肉缩的分子机制对于开发支持性疗法至关重要.

研究的目的:

在细胞模型中,研究大麻 (CBD) 是否可以预防西斯普拉丁诱导的肌肉缩.
探索CBD对蛋白质稳态,氧化应激和线粒体功能在对西斯普拉丁的反应中的影响.

主要方法:

使用了C2C12菌根管的体外模型,用西斯和大麻素 (CBD) 处理.
评估了肌管直径,蛋白质合成和降解速率,氧化应激标志物 (TBARS,催化酶),基因表达 (GPX1,SOD1,SOD2,CAT) 和线粒体蛋白质水平.

主要成果:

西斯普拉丁显著降低了肌管直径,改变了蛋白质平衡,而CBD联合治疗反转了这一现象.
CBD使氧化应激标志物中西斯普拉丁诱导的增加正常化,并纠正了抗氧化剂和线粒体基因的表达.
与CBD联合治疗恢复了受西斯普拉丁影响的线粒体蛋白质水平,表明对线粒体功能障碍和亡的保护.

结论:

大麻素 (CBD) 在体外有效地对抗西斯普拉丁诱导的肌肉缩.
CBD显示出作为癌症缓解症的辅助疗法的潜力,有助于肌肉质量保存和改善患者的生活质量.

关键词:

卡赫西亚 (Cachexia) 是一种缓解症.内分泌大麻素系统内分泌大麻素系统线粒体功能线粒体功能氧化应激是一种氧化应激.蛋白质的平衡是蛋白质的平衡.

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