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Updated: Jul 24, 2026

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood
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一套查技术,快速概述中性粒细胞功能

Isabelle Souza Luz1, Raquel Takaya1, Daiane Gonzaga Ribeiro1

  • 1Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, University of Brasilia.

Journal of visualized experiments : JoVE
|February 26, 2024
PubMed
概括

研究人员开发了NeutroFun Screen,这是一种快速的4小时测定,用于评估多种中性粒细胞功能,包括细胞活力,反应性氧物种 (ROS) 生产,迁移和细胞化. 这种全面的查有助于指导进一步的免疫学研究.

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Simple Staining Technique

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