改进单步三明治电化学ELISA的设计特征和气泡操纵技术,将商业电极纳入毛细血管流动驱动的免疫测试设备中
Phuritat Kaewarsa1, Melissa S Schenkel2, Kira L Rahn2
1Graduate Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.
The Analyst
|February 26, 2024
概括
这项研究介绍了iceCaDI设备,它是一种便携式,无的免疫测试系统,使用毛细血管作用和商业电极进行快速,单步电化学酶链接免疫吸收测试 (ELISA) 在护理点.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 电化学 电化学 电化学
背景情况:
- 定量点检测 (POCT) 从将电化学整合到毛细血管流动驱动的免疫检测设备中获益.
- 定制电极提供调整性,但需要复杂的制造.
- 现有的层状设备制造方法具有运行限制.
研究的目的:
- 开发一种简化,用户友好的毛细血管流动驱动免疫测试 (iceCaDI) 装置,用于医院电化学测试.
- 将商业电极集成到微流体装置中,用于单步三明治电化学酶相关免疫吸收试验 (ELISA).
- 为了解决微流体检测的层状设备制造和操作的局限性.
主要方法:
- 使用链制造可折叠的层状微流体装置,以便轻松组装和对齐.
- 采用箭头形的口,通过气泡波器有效分散试剂.
- 增加了通风孔,以防止气泡阻碍毛细血管流动.
- 集成商业印电极用于电化学检测.
主要成果:
- 在iceCaDI设备上成功制造了一个概念验证CRP (C-反应蛋白) 免疫传感器.
- 该设备显示CRP的检测范围为0.625到10.0μg mL-1.
- 在自动毛细管流条件下,在8分钟内精确测量了三种CRP度.
结论:
- 冰CaDI设备为开发先进的护理点 (POC) 诊断工具提供了一个有希望的方法.
- 这种无,便携式微流体系统简化了最终用户复杂的免疫测试.
- 开发的设备代表了向临床诊断的可访问的电化学免疫测试迈出的重要一步.
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