植物菌群中的III型多基酸合成酶集群参与了基酸醇生物合成
Lars Milke1, Moses Kabuu2, Renè Zschoche2
1Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich, 52425, Jülich, Germany.
Applied microbiology and biotechnology
|February 26, 2024
概括
浮游生物菌细菌具有III型多基酸合成酶 (PKS),可产生基酸,可能与皮科亚诺细菌化合物有关. 这些发现为发现这些未被探索的细菌中的新生物活性分子开辟了新的途径.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 生物信息学是一种生物信息学.
背景情况:
- 细菌系Planctomycetota是新兴生物活性化合物的新兴来源.
- 最近的生物勘探研究开始揭示这个族群中的化学多样性.
- 第三种类型的多基合成酶 (PKS) 是天然产品生物合成中的关键酶,产生具有生物活性的多种化合物.
研究的目的:
- 通过生物信息分析,系统地识别所有131种描述的Planctomycetota物种中的III型PKS基因.
- 调查这些PKS与已知的天然产品的潜在功能和生态联系.
- 探索已识别的 Planctomycetal III 类型 PKS 基因对化合物生物合成的异质表达.
主要方法:
- 对131个植物菌群基因组进行全基因组生物信息分析,以识别III型PKS基因.
- 遗传学分析以推断进化和功能关系.
- 在工程 *Corynebacterium glutamicum* 菌株中,选择的 Planctomycetal III 类型 PKS 基因的异质表达.
- 生物合成和由此产生的天然产品的特征.
- 现型测试用于确定合成化合物的生物功能.
主要成果:
- 在Planctomycetota基因组中确定了96个假定的III型PKS基因.
- 发现了58个组织在氧化还原酶和甲基转移酶基因的操作子中的III型PKS基因,表明与hierridins的联系.
- 在*C. glutamicum*中成功表达了Planctomycetal III型PKS基因,从而导致pentadecyl和heptadecylresorcinols的生物合成.
- 与其他细菌基复合素生产者相比,这些化合物具有明显的自然功能.
结论:
- 浮游生物群港口型III PKS 能够合成长链基复合素.
- 遗传学和遗传学证据表明,浮游生物PKS产物与皮科亚诺细菌的hierridins之间存在生态联系.
- *Corynebacterium glutamicum* 作为一个适合表达 Planctomycete 衍生基因的宿主,方便研究它们的生物合成途径和产物.
相关概念视频
The Photochemical Reaction Center
4.1K
Reaction centers are pigment-protein complexes that initiate energy conversion from photons to chemical entities. Therefore, photochemical reaction center is a more appropriate term that describes these complexes. The Nobel laureates Robert Emerson and William Arnold provided the first experimental evidence of photochemical reaction centers by demonstrating the participation of nearly 2,500 chlorophyll molecules for the release of just one molecule of oxygen. Despite thousands of photosynthetic...
4.1K
Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
The Calvin Benson Cycle
4.5K
Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...
4.5K
The Calvin Cycle
74.1K
Overview
74.1K
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
[3,3] Sigmatropic Rearrangement of Allyl Vinyl Ethers: Claisen Rearrangement
2.1K
The Claisen rearrangement is a [3,3] sigmatropic rearrangement of allyl vinyl ethers to unsaturated carbonyl compounds. The rearrangement is a concerted pericyclic reaction proceeding via a chair-like transition state.
2.1K


