氨引起的氧化应激引发了剃须 (Sinonovacula constricta) 的亡
Ziqi Guo1, Yukuan Chen1,2, Xinxin Du1
1Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai, 201306, China.
Environmental science and pollution research international
|February 26, 2024
概括
暴露于氨酸会通过破坏它们的抗氧化剂系统并触发亡来伤害贝类. 这些效应即使在恢复后仍然存在,突出的是氨.
科学领域:
- 水生物质毒理学研究
- 环境科学环境科学
- 海洋生物学 海洋生物学
背景情况:
- 氨是一种主要的水产养殖污染物,影响水生动物的健康.
- 贝类很容易受到氨对发育,新陈代谢和生存的影响.
- 有限的数据存在于贝类的免疫,抗氧化和亡反应对氨气压力.
研究的目的:
- 研究氨气压力对剃须免疫和抗氧化剂系统的影响.
- 在暴露于氨水下,分析剃须组织中的亡反应.
- 确定这些系统在氨压力后的恢复模式.
主要方法:
- 剃刀 (Sinonovacula constricta) 暴露在0.25 mg/L和2.5 mg/L的非离子氨中72小时.
- 暴露后在无氨海水中恢复72小时.
- 评估了抗氧化酶活动,活性氧物种水平,亡 (TUNEL染色) 和基因表达 (Caspase 7,Cyt-c,Bcl-2,Bax).
主要成果:
- 高度的氨破坏了抗氧化剂系统,增加了活性氧物种和改变了酶活动.
- 抗氧化剂系统的干扰和酶活性升高在康复72小时后仍然存在.
- 氨气压力显著改变了在消化腺和中的线粒体亡途径基因表达,没有完全恢复.
- 亡在上更为明显,表现出剂量和时间依赖的模式.
结论:
- 暴露于氨酸会激活剃须刀的先天免疫力.
- 氨压力破坏了抗氧化剂系统,并激活了线粒体的亡途径.
- 这些发现对于了解类贝类中氨的水生毒性机制至关重要.
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