从人类临床埃舍里希亚大肠杆菌分离物中对mcr-1-含有等离体的新见解
Florencia Martino1,2, Alejandro Petroni1, María Alejandra Menocal1
1Servicio Antimicrobianos, National Reference Laboratory in Antimicrobial Resistance (NRLAR), National Institute of Infectious Diseases (INEI), ANLIS "Dr. Carlos G. Malbrán", Buenos Aires City, Argentina.
PloS one
|February 26, 2024
概括
大肠杆菌中的移动胆固醇抗性 (mcr) 基因是一个越来越令人担忧的问题. 这项研究揭示了在阿根廷携带mcr-1的常见Inci2组之外的多种类型的等离子体,包括新的组合.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 传染性疾病 传染性疾病
背景情况:
- 移动胆固醇耐药性 (mcr) 基因正在格兰阴性细菌中出现,特别是在耐卡巴胺的肠道细菌中.
- 携带mcr-1基因的大肠杆菌菌是一种常见的组合,此前在阿根廷的表征仅限于Inci2等离子体.
研究的目的:
- 从阿根廷的临床大肠杆菌分离物中研究对mcr-1-含有等离体的新见解.
- 描述与mcr-1相关的等离子体骨干和抗性基因组合的多样性.
主要方法:
- 八个mcr-1-阳性大肠杆菌临床分离物的全基因组测序.
- 等离子体分析包括类型 (Inc 组) 和抗性基因的识别.
- 对大肠杆菌序列类型 (ST) 的遗传学分析.
主要成果:
- 确定了八种不同的mcr-1-含有塑体,属于Inci2,IncX4,Inci2/HI2A,以及一种新的InciFIA/HI1A/HI1B混合型.
- 这是阿根廷在Inci2集团之外首次报告mcr-1等离子体.
- 在四个不同Inc组的等离子体中发现了mcr-1.5变异,主要是在阿根廷.
- mcr-1与其他耐药基因的同时发生,包括tet(A),floR和各种CTX-M变体 (blaCTX-M-2,blaCTX-M-65,blaCTX-M-14).
结论:
- 这项研究扩大了对阿根廷大肠杆菌mcr-1等离子体多样性的理解,揭示了超出Inci2.2的多种等离子体类型.
- 这些发现突显了新型等离子体介导的素抗性机制和抗性基因组合的潜力.
- 持续监测至关重要,以监测各种mcr-1-含有等离子体和相关耐药基因的传播.
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