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一个保存的试索马提德分化调节器控制了Trypanosoma congolense中的基质附着和形态发展

Eleanor Silvester¹, Balazs Szoor¹, Alasdair Ivens¹

¹Institute for Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Ashworth laboratories, Charlotte Auerbach Road, Edinburgh, United Kingdom.

|February 26, 2024

概括

在Trypanosoma congolense中的调节器TcREG9.1控制了寄生虫的附着和发展. 沉默这个基因会导致脱落,影响牲畜的试索索菌传播.

科学领域:

寄生虫学的寄生虫学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

包括Trypanosoma congolense在内的Trypanosomatid寄生虫表现出对生命周期适应的发育调节.
来自Trypanosoma brucei的一种调节器TbREG9.1在Trypanosoma congolense中具有正义同类 (TcREG9.1),尽管后者缺乏的形态型.
三是牲畜疾病的重要原因之一.

研究的目的:

为了研究TcREG9.1在Trypanosoma congolense中的功能.
了解TcREG9.1在寄生虫附着,发育和传播中的作用.

主要方法:

通过RNA干扰 (RNAi) 介导的TcREG9.1.1.的基因沉默.
在体外测试测量寄生虫附着在基板上的测量.
现型分析包括固酶抑制剂治疗.
RNA测序 (RNAseq) 用于分析基因表达变化.
在体内研究以评估寄生虫水平和附着.

主要成果:

在实验室中,tcREG9.1基因沉默导致寄生虫从表面脱离.
脱离被模拟为固酶抑制剂治疗.
RNAseq揭示了表面分子mRNAs的上调,包括转移素受体样分子.
在体内,TCREG9.1的减弱导致了由于微血管粘附减少而导致寄生病的增加.
寄生虫的形态进展到昆虫的形式被打乱了.

结论:

TcREG9.1作为Trypanosoma congolense中附着和发育的关键调节器.
被TcREG9.1调节的脱离寄生虫可能适应传播.
这一发现提供了对畜牧动物试虫病和寄生虫传播的生物学见解.

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