相关实验视频

Updated: Jul 2, 2025

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

Published on: February 28, 2025

在Mycobacterium结核病感染中的类固醇免疫响应基因调节 in vitro

Maria Eduarda de Albuquerque Borborema¹, Débora Elienai de Oliveira Miranda¹, Thays Maria Costa de Lucena¹

¹Laboratory of Human Genetics and Molecular Biology, Genetics Department, Federal University of Pernambuco, Recife, Pernambuco, Brazil; Laboratory of Immunopathology Keizo Asami, Federal University of Pernambuco, Recife, Pernambuco, Brazil.

Tuberculosis (Edinburgh, Scotland)

|February 26, 2024

概括

维生素D3和17β-雌激醇调节了对结核病 (TB) 的免疫反应. 这些类固醇激素影响了Mycobacterium结核病感染细胞中的炎症基因表达和细胞因子释放.

科学领域:

免疫学免疫学免疫学
内分泌学在内分泌学.
传染性疾病传染性疾病

背景情况:

结核病 (TB) 是一种由Mycobacterium tuberculosis (Mtb) 引起的复杂传染病.
像维生素D3 (VD3) 和17β-雌二醇 (E2) 这样的类固醇激素具有与Mtb感染相关的免疫调节特性.
了解VD3和E2在Mtb感染期间对免疫基因表达的影响至关重要.

研究的目的:

研究VD3和E2对免疫和炎症标志物的基因表达在MTB的反应中的体外影响.
分析这些激素对人体外周血液单核细胞 (PBMCs) 中的细胞因子产生和Mtb活力的影响.

主要方法:

人类PBMCs用VD3或E2进行预处理,然后与Mtb (H37Rv菌株) 或脂多糖 (LPS) 共培养.
对NLRP3,DC-SIGN,IL-1β和IL-10的基因表达分析使用RT-qPCR在24小时和72小时进行.
在超级生物中测量了细胞因子水平 (IL-10,TNF,IFN-γ,IL-4,IL-6,IL-2),以及巨细胞中的Mtb活力.

主要成果:

VD3和E2都降低了Mtb联合培养细胞中NLRP3,IL-1β和IL-10炎症基因的表达.
VD3治疗增强了Mtb感染细胞中干扰素 (IFN-γ) 的释放.
治疗E2抑制了IL-10,瘤坏死因子 (TNF),IFN-γ和IL-6的释放.

结论:

在Mtb共同培养期间,VD3和E2对细胞反应产生免疫遗传影响.
这些发现突出了VD3和E2在调节宿主免疫力对结核病的潜在治疗作用.

关键词:

雌激素是一种雌激素.模式识别受体的模式识别受体结核病是一种疾病.维生素D ((3)) 的情况.

更多相关视频

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis

Published on: April 5, 2017

An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis

An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis

Published on: August 16, 2021

相关概念视频

Defense Against Bacterial Pathogens

Defense Against Bacterial Pathogens

The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...