核突变和适应性进化改善了Euglena gracilis的光自营生长,并固定了烟气CO2
Kai Xin1, Jun Cheng2, Ruhan Guo1
1State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, Zhejiang, China.
Bioresource technology
|February 26, 2024
概括
改造后的Euglena gracilis (M800) 显示了增强的生物质生长和二氧化碳固定. 这种微藻突变体表现出改善的RuBisCO活动和光合作用效率,为碳捕获应用提供了潜力.
科学领域:
- 微藻生物技术 微藻生物技术
- 光合作用碳固定的光合作用.
- 应变工程是一种应变工程.
背景情况:
- 微藻对于生物质生产和二氧化碳捕获至关重要.
- 提高微藻生长和二氧化碳固定效率对于工业应用至关重要.
- 耐酸性Euglena gracilis提供了用于烟气二氧化碳利用的潜力.
研究的目的:
- 为了增强Euglena gracilis中的生物质增长和二氧化碳固定.
- 开发一种强大的微藻菌株,用于工业碳捕获.
- 研究提高性能背后的生理和分子机制.
主要方法:
- -60玛射线辐射用于突变发生.
- 聚乙烯甘醇 (PEG) 适应性选用于突变选择.
- 测量生物质干重量,二氧化碳固定率,RuBisCO活性和光合作用颜料.
- 评估抗氧化能力和细胞亡/亡率.
主要成果:
- 突变菌株M800的生物质干重和二氧化碳固定率比野生类型高1.47倍.
- M800显示RuBisCO活性和光合作用色素含量增加.
- 在PSII中观察到增强的电荷分离量子效率,高效的光利用和能量调节在M800.
- M800表现出更强的抗氧化能力和降低脂质过氧化.
- 用1毫米的PEG补充剂进一步增加了M800生物量79.1%,促进了细胞增殖,同时降低了细胞亡和亡.
结论:
- 经过工程改造的Euglena gracilis M800菌株显著提高了生物质产量和二氧化碳固定.
- 增强的光合作用效率,RuBisCO活性和抗氧化能力是M800卓越性能的基础.
- PEG应用进一步提高了M800的增长和可行性,突出了其大规模碳捕获技术的潜力.
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