毒性和多抗药性等离子体在尿病原菌大肠杆菌中的共存
Yongli Wu1, Ziyao Li2, Zichen Lei2
1Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China; Laboratory of Clinical Microbiology and Infectious Diseases, Department of Pulmonary and Critical Care Medicine, National Center for Respiratory Medicine, China-Japan Friendship Hospital, Beijing, China; Institute of Respiratory Medicine, Chinese Academy of Medical Sciences, Beijing, China.
Journal of global antimicrobial resistance
|February 26, 2024
概括
这项研究报告了一种来自中国的多抗药性大肠杆菌菌株. 该菌株含有塑体,使其具有抗生素和毒性因子的耐药性,这凸显了监测这种病原体的必要性.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 具有多个移动耐药性和毒性元素的病原体的出现构成了临床威胁.
- 大肠杆菌菌株是尿路感染的重要原因.
研究的目的:
- 在中国从尿路感染中分离出来的O101:H10-ST167大肠杆菌菌株 (Hu106) 的特征.
- 阐明大肠杆菌Hu106菌株的抗微生物耐药性,毒性机制和遗传元素.
主要方法:
- 对抗生素敏感性测试以确定耐药性谱.
- 全基因组测序 (WGS) 和生物信息分析以确定耐药性和病毒性基因.
- 大幼虫测定和 siderophore 生产实验以评估毒性.
主要成果:
- 大肠杆菌Hu106对大多数经过测试的抗微生物药物表现出耐药性,仅对阿兹特雷诺姆,阿米卡辛和提格环素敏感.
- WGS揭示了blaNDM-9和mcr-1在等离子体p2-Hu106 (IncHI2/IncHI2A) 上的共同存在,通过转移重组获得.
- 在等离子体p1-Hu106 (IncFIB/IncFIC(IncFII) 上鉴定出毒性因子aerobactin和salmochelin,这些因子显示出在肠杆菌中传播的潜力. 这种菌株的幼虫存活率降低, siderophore 生产量高.
结论:
- 大肠杆菌Hu106菌株既具有多药性耐药性,也具有毒性等离子体.
- 监测携带这些等离子体的菌株对于防止进一步传播和减轻临床风险至关重要.
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