从新鲜脂肪组织或在夜间储存后分离出来的人类动脉膜的蛋白质组概况
Rajan Pandey1, Michelle L Roberts2, Jingli Wang2
1Center of Systems Molecular Medicine, Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin; Department of Physiology, University of Arizona College of Medicine-Tucson, Tucson, Arizona.
Laboratory investigation; a journal of technical methods and pathology
|February 26, 2024
概括
人类动脉小管的蛋白质组分析现在是可行的,即使是小样本和一夜冷藏. 这一突破为研究血压和动脉相关特征提供了有价值的参考蛋白质.
科学领域:
- 血管生物学 血管生物学
- 蛋白质组学是指蛋白质组学.
- 生物化学 生物化学
背景情况:
- 动脉管对于调节血压至关重要.
- 从孤立的人类动脉小管中获得的低蛋白质产量阻碍了蛋白质组分析.
- 手动剖析和运输临床标本对研究构成后勤挑战.
研究的目的:
- 为了克服动脉蛋白质组学中的低输入限制.
- 为了评估人类动脉小动脉组织的夜间冷藏.
- 建立一个全面的人类动脉动脉蛋白质基因参考资料.
主要方法:
- 利用了双重质量标签 (TMT) 蛋白质组学与干细胞衍生细胞的助推通道.
- 在冷藏后和运输过程中评估蛋白质质谱的一致性.
- 分析了来自3个受试者的12个动脉样本,以构建参考蛋白质组.
主要成果:
- 一个助推道将检测到的蛋白质从每样本的1051增加到3000多个.
- 在过夜冷藏后,蛋白质组形状保持一致.
- 一个参考人脉动脉蛋白质组确定了3832种蛋白质,其中包括476种与血压相关的蛋白质.
结论:
- 使用低输入样本和过夜冷藏,对人类动脉小动脉的蛋白质组分析是可行的.
- 发达的人类动脉蛋白质组是研究的宝贵资源.
- 这些发现支持了多中心研究关于动脉小板相关特征和血压调节的研究.
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