Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Mutations01:39

Mutations

82.2K
Overview
82.2K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

6.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
6.8K
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

4.8K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.8K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

7.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.0K
Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

5.7K
Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.7K
RNA Splicing01:32

RNA Splicing

56.4K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

High-throughput screening identifies a critical role of the SPOP-PABPC1 axis in lung adenocarcinoma progression.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

TP53 mutations in triple-negative breast cancer cells confer sensitivity to ASCT2 inhibition via arginine uptake.

Cell death & disease·2026
Same author

Explicit differentiable slicing and global deformation for cardiac mesh reconstruction.

Medical image analysis·2026
Same author

Trends and lifestyle contributions to frailty in US adults aged 50 and older: analysis of health and retirement study data (2004-2020).

BMC public health·2026
Same author

Reversible acetylation of Hpo regulates the Hippo pathway.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Remodelling membrane lipid composition through exogenous supplementation of stearic acid improves the survival rate of spray-dried Bifidobacterium animalis.

Food research international (Ottawa, Ont.)·2026
Same journal

Small molecule activators of the mitochondrial protease ClpP induce senescence in triple-negative breast cancer cells and sensitize cells to the Bcl-2 inhibitor venetoclax.

Cell death & disease·2026
Same journal

Targeting EZH2-driven cholesterol metabolic vulnerability through Napabucasin suppresses ovarian cancer metastasis.

Cell death & disease·2026
Same journal

Knockdown of endothelial Serpine1 improves stroke recovery by attenuating peri-infarct blood flow and blood-brain barrier disruption.

Cell death & disease·2026
Same journal

EGFR-dependent delivery of phosphorylated IGFBP3 by cancer stem cells drives chemotherapy resistance in colorectal cancer.

Cell death & disease·2026
Same journal

TRMT1-mediated tRNA m<sup>2</sup><sub>2</sub>G modification drives Osimertinib resistance via the ATXN3/USP25 axis in lung adenocarcinoma.

Cell death & disease·2026
Same journal

Dynamic S-palmitoylation of glioma-associated oncogene 1 regulates the incidence and progression of Sonic Hedgehog medulloblastoma.

Cell death & disease·2026
查看所有相关文章

相关实验视频

Updated: Jul 2, 2025

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

2.5K

SPOP点突变调节基质偏好并影响其功能.

Yanran Deng1,2, Wenhao Ding3, Kaize Ma3

  • 1Jiangsu Key laboratory of Drug Screening, China Pharmaceutical University, 210009, Nanjing, China.

Cell death & disease
|February 26, 2024
PubMed
概括
此摘要是机器生成的。

该研究确定IRF2BP2是SPOP的基质,SPOP是一种参与癌症的蛋白质. 矛盾的是,SPOP中的突变可以通过改变其与IRF2BP2的相互作用来促进癌症.

更多相关视频

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

9.6K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

2.7K

相关实验视频

Last Updated: Jul 2, 2025

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

2.5K
Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

9.6K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

2.7K

科学领域:

  • 分子生物学分子生物学
  • 在瘤学瘤学.
  • 生物化学 生物化学

背景情况:

  • 该SPOP蛋白充当适应器,为CUL3 E3结合酶招募基质进行无处不在和降解.
  • SPOP拥有用于基质识别的MATH域和用于CUL3绑定的BTB域.
  • 在SPOP的MATH域中的突变与瘤发生有关,可能是通过破坏基质相互作用.

研究的目的:

  • 确定SPOP的新基质,并研究SPOP突变在肝细胞癌 (HCC) 的功能后果.
  • 探索SPOP及其突变形式在HCC中调节细胞增殖和转移中的作用.

主要方法:

  • 鉴定IRF2BP2作为SPOP的新基质.
  • 分析SPOP在减弱HCC细胞中IRF2BP2介导作用中的作用.
  • 来自HCC的SPOP突变 (SPOP-M35L) 的特征及其与IRF2BP2的亲和力2.

主要成果:

  • 通过向IRF2BP2.2,SPOP可以抑制HCC细胞的增殖和转移.
  • 野生型SPOP的过度表达抑制了HCC细胞的增殖和转移.
  • 这种SPOP-M35L突变对IRF2BP2具有增加的亲和力,并促进HCC细胞的增殖和转移,这表明从瘤抑制剂切换到coprotein.

结论:

  • 在SPOP的MATH域中发生的突变具有上下文依赖的功能后果,不仅仅是干扰基质相互作用.
  • 该SPOP-M35L突变可能会重编程SPOP的功能,促进HCC中的瘤发生.
  • 在考虑SPOP作为癌症的治疗点时,建议谨慎,因为其突变的复杂和上下文依赖的影响.