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静止使天真胚胎干细胞中不受限制的细胞命运成为可能
Le Tran Phuc Khoa1,2, Wentao Yang1, Mengrou Shan2
1Department of Medicine, Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA, 90033, USA.
Nature communications
|February 26, 2024
概括
静止干细胞不是休眠的,而是具有不受限制的细胞命运,能够形成胚胎和胚胎外血统. 这种过渡涉及代谢转变和表观遗传变化,揭示了对干细胞可塑性的新见解.
科学领域:
- 干细胞生物学 干细胞生物学
- 发育生物学是发展生物学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 干细胞中的静止通常被视为休眠或平衡的潜力.
- 幼稚的小鼠胚胎干细胞 (ESCs) 可以进入静止状态,模仿胚胎断.
- 静止ESCs (qESCs) 的分子机制和发展潜力在很大程度上是未知的.
研究的目的:
- 为了研究qESCs的分子基础.
- 探索qESCs的发展潜力.
- 了解qESCs的代谢和表观遗传情景.
主要方法:
- 在小鼠ESC中诱导静止.
- 细胞命运和发育潜力的分析 (例如, trofhoblast 干细胞的生成).
- 代谢分析,H3K27me3的ChIP-seq,ATAC-seq和基因表达分析.
- 基因操纵 (Mat2a消耗,中国人民共和国的Eed删除2).
主要成果:
- qESCs表现出扩展的细胞命运,产生胚胎和胚胎外细胞类型.
- qESCs表现出明显的代谢特征,包括降低S-adenosylmethionine.
- 代谢变化与减少的H3K27me3,增加的染色质可访问性和MERVL和 trofhoblast调节者的抑制相关.
- 操纵 metionin代谢或PRC2会影响向外胚胎血统的发展潜力.
结论:
- 安静的社会经济委员会正在积极过渡,而不是休眠.
- 静止赋予细胞不受限制的命运潜力.
- 代谢和表观遗传重编程驱动了qESCs中的这种过渡.
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