编码和非编码调节之间的相互作用驱动了Arabidopsis从种子到种子的过渡
Benjamin J M Tremblay1, Cristina P Santini1, Yajiao Cheng1
1Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra, Barcelona, Spain.
Nature communications
|February 26, 2024
概括
种子发芽依赖于mRNA翻译,但当RNA合成开始时尚不清楚. 这项研究表明,活跃的转录发生在整个发芽过程中,揭示了广泛的非编码RNA调节及其在植物发育中的作用.
科学领域:
- 植物分子生物学 植物分子生物学
- 转录法规 转录法规
- 种子的发芽 种子的发芽
背景情况:
- 种子发芽需要存储的mRNAs的翻译.
- 在种子到种子过渡期间,RNA聚合酶II (RNAPII) 在RNA合成中重新参与的精确时间尚未完全理解.
- 非编码RNA在基因调节中起着至关重要的作用.
研究的目的:
- 调查种子发芽期间活跃转录启动的动态.
- 描述非编码RNA和染色质可访问性在发芽期间调节基因表达中的作用.
- 探索在这个发育阶段对酸 (ABA) 敏感性的机制.
主要方法:
- 结合的计算小RNA测序 (csRNA-seq),测序 (ATAC-seq) 的转移酶可访问染色体的测试,以及单分子光现场混合 (smFISH).
- 使用了Arabidopsis thaliana作为模型生物.
- 在关键发现中使用遗传验证.
主要成果:
- 在Arabidopsis.在整个发芽过程中,可以检测到活跃的转录启动.
- 观察到广泛的非编码RNA调节,包括反意义转录和双向促进剂.
- 发芽对外源性酸 (ABA) 的敏感性与对ABA敏感基因的近位促进物可访问性有关.
- 不同的转录和转录的增强元件产生增强RNA (eRNA) 被遗传验证.
结论:
- 本研究定义了关键发芽阶段编码和非编码转录的范围和功能.
- 这些发现扩大了对管理植物发育过渡的转录机制的理解.
- 活跃转录和非编码的调节元素是成功种子发芽的组成部分.
相关概念视频
02:24Regulation of Expression Occurs at Multiple Steps
3.0K
3.0K
01:10Transcription
147.0K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
147.0K
01:23Regulation of Expression at Multiple Steps
907
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
907
02:33Seed Structure and Early Development of the Sporophyte
28.2K
Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.
28.2K
01:25Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
02:02Cis-regulatory Sequences
9.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.9K