第一次对致病性Leptospira spp.进行分离和基因型鉴定. 来自奥地利的来自奥地利
Cynthia Sohm1,2,3, Denise Willixhofer4, Eva Fasching4
1VetFarm, Department of Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Kremesberg 13, 2563, Pottenstein, Austria. cynthia.sohm@vetmeduni.ac.at.
Scientific reports
|February 26, 2024
概括
研究人员从奥地利牛中分离了当地Leptospira菌株,识别了Leptospira borgpetersenii. 这一突破有助于改善欧洲的白病诊断和动物传播疾病流行病学的理解.
科学领域:
- 兽医微生物学 兽医微生物学
- 动物传播疾病流行病学
- 公共卫生 公共卫生
背景情况:
- 莱普托斯皮罗斯是一种全球性动物传播性疾病,具有诊断挑战.
- 标准的微观聚合试验 (MAT) 需要活的LeptoSpira菌株.
- 当地菌株对于提高MAT敏感性和特异性至关重要,但在奥地利没有任何可用菌株.
研究的目的:
- 从奥地利牛群中分离和鉴定流传中的Leptospira菌株.
- 为了提高在奥地利对人类和动物的常规白病血清学测试的性能.
- 为了有助于了解欧洲的白螺旋菌病流行病学.
主要方法:
- 统计分析和文献审查确定了高风险的牛群.
- 从410头牛 (2021年11月至2022年10月) 取尿液和脏组织的有针对性的抽样.
- 实时PCR用于致病性Leptospira (lipL32基因) 和核心基因组多部位序列类型 (cgMLST) 进行隔离特征.
主要成果:
- 在429个测试样本中,有9个对致病性LeptoSpira的PCR阳性.
- 三个Leptospira分离物成功培养和鉴定.
- 分离物被证实为Leptospira borgpetersenii血清组Sejroe血清组Hardjobovis,cgMLST集群40的分离物.
结论:
- 这项研究报告了在奥地利首次隔离和基因定型本地动物性传染病Leptospira.
- 确定的本地菌株虽然适应牛,但具有显著的动物感染影响.
- 这些发现有可能改善奥地利的白病诊断性能,并增强欧洲的流行病学知识.
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