Twist-1通过PI3K/AKT路径刺激水形分子的恶性行为
Chengling Zhang1, Yan Song1, Yanxue Yin2
1Department of Gynecology, Jinan Maternity and Child Care Hospital, 250001 Jinan, Shandong, China.
Discovery medicine
|February 27, 2024
概括
沉默Twist-1,一种涉及瘤的蛋白质,可以减少水性形和胆癌细胞的生长,迁移和入侵. 这表明Twist-1抑制可以通过影响上皮质-介质酶过渡和PI3K/AKT通路来作为治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 妇科 妇科医生 妇科
背景情况:
- 水性形 (HM) 是一种常见的妊娠并发症.
- 与Twist相关的蛋白1 (Twist-1) 在HM病变发生过程中的作用尚不清楚.
- 这项研究研究了Twist-1在HM和胆瘤 (CCA) 细胞中的表达和功能.
研究的目的:
- 为了确定Twist-1表达水平在水性形体组织和胆管癌细胞中.
- 阐明Twist-1在胆管癌细胞增殖,迁移和入侵中的生物学功能.
- 探索Twist-1沉默对上皮质-介质酶过渡 (EMT) 和PI3K/AKT信号通路的影响.
主要方法:
- 使用免疫组织化学和qRT-PCR来评估Twist-1表达.
- 在Twist-1沉默后,通过CCK-8和殖民地形成试验来评估细胞增殖.
- 使用Transwell试验分析了细胞迁移和入侵.
- 西方涂抹被用于检查EMT标记物和PI3K/AKT通路蛋白.
主要成果:
- 在HM组织和CCA细胞中,Twist-1表达显著上调.
- 沉默Twist-1显著抑制了BeWo和JAR细胞的增殖,迁移和入侵.
- 扭曲-1敲击促进了E-cadherin的表达,同时抑制了N-cadherin和vimentin.
- 对Twist-1的下调降了PI3K和AKT蛋白质的酸化水平.
结论:
- 在水性形和胆管癌中,Twist-1被上调.
- 沉默Twist-1可以抑制胆管癌细胞的恶性行为.
- 扭曲-1抑制可以通过抑制EMT和PI3K/AKT通路来发挥其作用.
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