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CircSEPT9通过通过海绵化miR-625-5p调节PTBP3表达来促进乳腺癌的进展

Guangtao Shi¹, Hongbo Li², Ying Chen²

¹Zhejiang Society for Mathematical Medicine, Hangzhou, China.

Thoracic cancer

|February 27, 2024

概括

循环RNA Septin 9 (circSEPT9) 通过通过海绵式微RNA-625-5p (miR-625-5p) 增加聚胺通道结合蛋白3 (PTBP3) 水平,促进乳腺癌 (BC) 的进展. 沉默circSEPT9可以抑制BC细胞的增殖和迁移.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

乳腺癌 (BC) 是一种影响妇女健康的流行恶性瘤.
循环RNAs (circRNAs) 在各种癌症中发挥着重要作用,包括BC.
了解BC进展中circRNAs的分子机制至关重要.

研究的目的:

研究circRNA Septin 9 (circSEPT9) 在乳腺癌进展中的作用和分子机制.
为了阐明circSEPT9,microRNA-625-5p (miR-625-5p) 和BC中的多胺管结合蛋白3 (PTBP3) 之间的相互作用.

主要方法:

定量实时PCR (qRT-PCR) 和西布洛特测试以评估分子水平.
细胞计数套件-8 (CCK8),EDU,流细胞计量,Transwell,以及用于功能分析的伤口愈合试验.
双露西法酶记者和RNA免疫沉 (RIP) 测试以确认相互作用;体内异种移植模型用于验证.

主要成果:

在BC组织和细胞中,circSEPT9过度表达.
circSEPT9 knockdown 抑制了BC细胞的增殖,迁移,上皮-介质细胞过渡 (EMT) 和糖解,同时促进了细胞亡.
circSEPT9针对miR-625-5p,miR-625-5p通过降低PTBP3.3的调节来调节BC细胞表型.

结论:

circSEPT9通过通过海绵化miR-625-5p对PTBP3进行上调来促进BC恶性进展.
circSEPT9作为BC发育和进展的关键调节者.
针对circSEPT9/miR-625-5p/PTBP3轴可能为BC提供治疗策略.

关键词:

在PTBP3中,PTBP3是PTBP3.乳腺癌乳腺癌乳腺癌 circSEPT9 circSEPT9 circSEPT9 circSEPT9 circSEPT9 circSEPT9 circSEPT9 circSEPT9 circSEPT9 在 miR-625-5p 中.

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