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短链脂肪酸propionate和butyrate控制与细胞代谢相关的生长和分化

Michael Nshanian¹, Benjamin S Geller¹, Joshua J Gruber¹

¹Department of Genetics, Stanford University, School of Medicine, Stanford, CA.

Research square

|February 27, 2024

概括

短链脂肪酸 (SCFA),如 propionate 和 butyrate 修改组织蛋白,影响基因表达. 这些修改促进细胞生长,分化和离子运输,以不同的方式影响正常和结直肠癌细胞.

科学领域:

生物化学生物化学
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

微生物的新陈代谢产生短链脂肪酸 (SCFA),如 propionate 和 butyrate.
SCFA 作为独特的乙 lysine 基因素标记起作用,影响细胞过程.
了解SCFA介导的表观遗传调节对于细胞生物学和疾病至关重要.

研究的目的:

为了绘制H3K18pr/bu和H4K12pr/bu基因组标记的全基因组位置.
研究这些SCFA修饰在结直肠癌 (CRC) 和正常细胞中的功能作用.
为了将SCFA标记与染色质可访问性和基因表达模式相关联.

主要方法:

染色体免疫沉测序 (ChIP-seq) 用于识别组织蛋白标记位置.
分析包括治疗和未治疗的CRC和正常细胞,以及小鼠肠道.
数据与开放的染色质区域和基因表达数据相关联.

主要成果:

在整个基因组中绘制了propionate和butyrate修改.
这些SCFA标记与开放的染色质有关,并影响基因表达.
发现SCFA促进细胞生长,分化和离子运输.

结论:

SCFA 基因组修饰直接改变特定的基因组区域,增加染色体的可访问性.
丁酸盐对正常细胞与CRC细胞的增殖具有相反的影响.
这些发现阐明了SCFA在表观遗传调节和细胞功能中的作用,对CRC有影响.

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