相关实验视频

Updated: Jul 2, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

在患有骨髓瘤恶性瘤风险的患者中进行TP53突变查

Devdeep Mukherjee¹, Rialnat A Lawal², Courtney D Fitzhugh²

¹Laboratory of Myeloid Malignancies, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD.

medRxiv : the preprint server for health sciences

|February 27, 2024

概括

在治疗前识别先前存在的TP53突变可以预测患上与治疗相关的髓状瘤的风险. 超深度DNA测序为这一关键风险评估提供了前性方法.

科学领域:

血液学血液学血液学
在瘤学瘤学.
遗传学是一个遗传学.

背景情况:

与治疗相关的髓状瘤恶性瘤是越来越令人担忧的,特别是在细胞疗法之后.
已经存在的TP53突变的造血克隆与其发展有关.
目前没有为高危患者进行前性查.

研究的目的:

建立一种方法来前性识别患有与治疗相关的骨髓质恶性瘤高风险的患者.
调查超深DNA测序对检测前恶性TP53突变的有用性.

主要方法:

在治疗前对患者样本进行潜在的超深度DNA测序.
分析测序数据以识别TP53突变.

主要成果:

超深度DNA测序可以检测事先存在的TP53突变.
检测到的TP53突变与随后恶性瘤的发展有关.

结论:

超深度DNA测序可以前性地识别患有与治疗相关的骨髓性恶性瘤高风险的患者.
这种方法可以早期检测与不良结果相关的TP53突变.
在启动细胞疗法之前,方便个性化风险评估.

更多相关视频

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

相关概念视频

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...