在Carassius auratus中与HPG轴相关的基因的表达和定位具有不同的 ploidy
Xiaowei Xu1, Li Yang1, Xinyi Deng1
1State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan, China.
Frontiers in endocrinology
|February 27, 2024
概括
三倍体十字鱼 (C. 甲状腺 (auratus) 在关键的生殖基因中表现出增强的基因表达和改变的DNA甲基化,支持正常的生殖腺发育和生育能力. 这项研究澄清了三鱼生育能力的遗传基础.
科学领域:
- 水生生物学 水生生物学
- 遗传学 遗传学 是一个
- 生殖生物学 生殖生物学
背景情况:
- 克鲁西安鱼 (C.C. 东水系统中的奥拉图斯 (auratus) 综合体包括双倍体和多倍体形式.
- 二倍体 (2nCC) 和三倍体 (3nCC) 鱼表现出相似的生育能力,在一岁时达到性成熟.
研究的目的:
- 与二倍体 (2nCC) 形式相比,研究三倍体鱼 (3nCC) 的生育能力背后的分子机制.
- 分析基因表达,3nCC和2nCC中关键的下丘脑-垂体-淋巴腺 (HPG) 轴基因的基因表达,DNA甲基化和蛋白质局部化.
主要方法:
- 分析核酸序列,基因表达水平,DNA甲基化模式和免疫光局部化.
- 专注于淋巴激素释放激素2 (Gnrh2),淋巴激素β (Gthβ) 和淋巴激素释放激素受体 (Gthr) 的基因.
主要成果:
- 与二倍体 (2nCC) 相比,三倍体十字鱼 (3nCC) 的拷贝数和Gnrh2,Fshr和Lhr基因的结构差异增加.
- 在3nCC中观察到HPG轴基因的更高的转录水平,这表明增强的性类固醇激素生产和淋巴腺发育.
- 在3nCC中HPG轴基因的促进子区域的较低DNA甲基化水平表明3nCC在三化后的基因表达中起着调节作用.
- 免疫光证实Fshβ,Lhβ和Fshr基因的局部不变,确保正常的表达部位.
结论:
- 这项研究提供了分子证据,证明了三倍体鱼的正常生殖腺发育和雌同体成熟.
- 这些发现有助于理解三形C. triploid中恢复生育能力的遗传基础. 奥拉图斯 (auratus) 是一个星座.
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