在PhoP合成的转录后控制中介的Salmonella病毒性基因的ProQ依赖激活
Sofia Bergman1, Liis Andresen1, Jonas Kjellin1
1Department of Cell and Molecular Biology, Biomedical Center, Uppsala University, Uppsala, Sweden.
mSphere
|February 27, 2024
概括
沙门氏菌使用RNA结合蛋白ProQ来控制其细胞内毒性. 这项研究揭示了ProQQ.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 细菌病原体的产生
背景情况:
- 沙门氏菌 (Salmonella enterica) 通过在宿主细胞内复制引起胃肠道疾病.
- 病毒性基因表达受转录和转录后的调节.
- RNA结合蛋白 (RBPs) 和小调节性RNA (sRNAs) 在转录后调节中发挥关键作用.
研究的目的:
- 在模仿含沙门氏菌真空体 (SCV) 的条件下,对RBP ProQ的全球RNA连接体谱进行表征.
- 阐明ProQ在调节沙门氏菌细胞内毒性计划中的作用,在转录后水平.
主要方法:
- 在SCV模仿条件下的高分辨率ProQ互动原子映射.
- 对ProQ依赖的sRNAs和mRNA 3'UTRs的分析.
- 评估ProQ对PhoP表达和下游毒性基因的影响.
主要成果:
- 在sRNA和mRNA3'UTR中发现了数百个新的ProQ结合位点.
- ProQ稳定了许多sRNA连接体,其中一些与Hfq相互作用.
- 在转录后,ProQ会激活PhoP的表达,从而提高细胞内毒性程序的调节.
结论:
- ProQ是沙门氏菌细胞内生活方式的关键调节者.
- ProQ的RNA结合活性对于控制病毒性基因表达在转录后至关重要.
- 向ProQ可能为打击沙门氏菌感染提供新的策略.
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