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优化选切断在头发中滥用药物的药物,使用免疫测试用于法医应用程序
Arianna Giorgetti1, Jennifer P Pascali1, Guido Pelletti1
1Department of Medical and Surgical Sciences, Unit of Legal Medicine, University of Bologna, Italy.
概括
优化的免疫测试 (IA) 查切断点用于头发分析,增强了法医毒理学. 头发检测中的负IA结果在法医上是有效的,减少了对确认性质谱 (MS) 分析的需求.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
背景情况:
- 阳性免疫测试 (IA) 结果需要在法医毒理学中进行质谱 (MS) 确认.
- 头发分析的负IA结果被认为是法医有效的,否定了进一步测试的需要.
研究的目的:
- 使用免疫试验 (IAs) 优化选切断点进行头发样本分析.
- 评估AI测试在头发样本中药物的法医实用性.
主要方法:
- 使用IA (ILab650) 分析了150名药物成患者的头发样本.
- 使用UPLC进行了确认分析.
- 使用接收器操作特征 (ROC) 分析来优化选切断.
主要成果:
- 针对安非他命,MDMA,可卡因,大麻素,甲和阿片类药物,建立了优化的选切线.
- 对所有测试药物来说,优化的切断值显示出高灵敏度和特异性 (AUC > 0.95).
- 在各种药物类别中确认了162个阳性结果.
结论:
- 优化的IA查方法在头发样本中有效检测常见滥用药物.
- 这种方法适用于意大利和欧洲的法医应用.
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