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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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相关实验视频

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Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
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在长时间尺度上观察ER动态,使用光板光显微镜.

Charlotte Pain1, Verena Kriechbaumer1, Alessia Candeo2,3

  • 1Endomembrane Structure and Function Research Group, Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, UK.

Methods in molecular biology (Clifton, N.J.)
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PubMed
概括

光板光显微镜 (LSFM) 能够实现植物发育的先进实时成像. 这项研究详细介绍了Arabidopsis叶中LSFM的协议,以观察热应激期间的内分泌网膜动态.

关键词:
阿拉比多普西斯 (Arabidopsis) 是一种植物.菲律宾 菲律宾 菲律宾在GFP-HDEL中使用.光板光显微镜 (LSFM) 的使用摄影漂白是一种照片漂白.光毒性 摄影毒性

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科学领域:

  • 植物生物学 植物生物学
  • 显微镜的使用方法
  • 细胞生物学 细胞生物学

背景情况:

  • 光板光显微镜 (LSFM) 已经彻底改变了多细胞生物的实时成像.
  • LSFM提供高分辨率,长期数据收集与最小的光损伤.
  • 它非常适合用于植物细胞生物学中的定量3D时间延迟成像.

研究的目的:

  • 介绍一个详细的LSFM协议,用于成像阿拉比多普西斯叶.
  • 评估LSFM的光毒性,并优化实验设置.
  • 为了监测内质网膜 (ER) 对热冲击的反应的动态.

主要方法:

  • 开发定制的支架和安装技术,用于阿拉比多普西斯的叶子.
  • 实施LSFM用于光标记ER标记器的实时成像.
  • 在受控热应激条件下量化动态细胞过程.

主要成果:

  • 建立了一个强大的LSFM协议,用于Arabidopsis叶片成像.
  • 评估和管理LSFM系统的光毒性.
  • 在热冲击期间ER结构的动态变化得到了成功监测.

结论:

  • LSFM是一种强大的技术,用于研究植物中的动态细胞过程.
  • 提出的协议有助于对植物有机体对环境压力的反应进行定量分析.
  • 这种方法提升了我们对植物细胞生物学和应激反应的理解.