一个模型的溶剂依赖性排放特性使得奥龙能够在生物应用中使用
Beth Anderson1, Daniel L Bryant2, Samer Gozem3
1Department of Chemistry, Middle Tennessee State University, 1301 East Main Street, Murfreesboro, TN, 37132, USA.
Journal of fluorescence
|February 27, 2024
概括
新的极光光体显示出作为启动探针的潜力. 瓦尼林紫外线光是由溶剂极性和蛋白质结合调节的,使其能够在水环境和细胞成像中应用.
科学领域:
- 有机化学 有机化学
- 摄影化学的使用.
- 生物物理化学 生物物理化学
背景情况:
- 小的有机光体对于可视化至关重要,而光子衍生物显示出有前途.
- 了解结构属性关系是开发新型光探针的关键.
研究的目的:
- 为了研究结构因素和溶剂环境如何影响瓦尼林 aurone 的光.
- 探索瓦尼林奥龙作为在水性介质中的启动探头的潜力.
主要方法:
- 在不同极性和介电常数的溶剂中表征氨酸光.
- 时间依赖密度函数理论 (TD-DFT) 计算以建模兴奋状态行为.
- 用ATP结合蛋白YME1L和细胞透性试验进行结合研究.
主要成果:
- 溶剂极性和键显著影响极光辐射特性 (波长,强度,斯托克斯转移).
- TD-DFT计算表明,水中扭曲的兴奋状态灭光,而非极性溶剂中的平面状态增强光.
- 瓦尼林奥龙在与YME1L (Kd ≈ 30μM) 结合时表现出开启探针的行为,并显示细胞透性,毒性超过6.25μM.
结论:
- 极光光对溶剂相互作用和分子构成非常敏感.
- 瓦尼林奥龙可以通过改变溶剂极性或通过宏分子结合在水性环境中充当启动探针.
- 紫外线代表了一种通用的支架,用于开发生物应用的新光探针.
相关概念视频
Atomic Emission Spectroscopy: Overview
2.2K
Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
2.2K
Atomic Emission Spectroscopy: Lab
162
AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
162
Atomic Emission Spectroscopy: Instrumentation
422
The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers. Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
422
Atomic Fluorescence Spectroscopy
327
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
327
Variables Affecting Phosphorescence and Fluorescence
501
Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
501
Photoluminescence: Applications
395
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
395


