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使用臭氧水力动力系统对人类胚胎膜进行绝育
Túlia Botelho1,2, Bianca Akemi Kawata3,4, Silvia Móbille Awoyama1,5
1Center for Innovation, Technology and Education - CITÉ, Parque de Inovação Tecnológica de São José dos Campos, São José dos Campos, SP, 12247-016, Brazil.
Annals of biomedical engineering
|February 27, 2024
概括
臭氧化动态水有效地在15分钟内对人类胎膜 (hAM) 进行对常见微生物的消毒,为组织工程支架提供了对马辐射的有希望的替代方案. 这种方法也显示出脱皮质化的潜力.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 微生物学 微生物学
背景情况:
- 人类羊水膜 (hAM) 是一个有价值的生物材料用于组织工程,因为它的再生特性.
- 传统的绝育方法,如马辐射,可以损害hAM结构和生长因子.
- 需要使用可替代的杀菌技术来保护hAM的完整性.
研究的目的:
- 评估臭氧化动态水对人类胎膜 (hAM) 灭菌的疗效.
- 评估臭氧化时间对微生物无活化和脱氧化的影响.
- 调查臭氧灭菌对hAM结构完整性的影响.
主要方法:
- 在实验中,hAM碎片被污染了黄金葡萄球菌,大肠杆菌,白菌,表皮葡萄球菌和Clostridium sporogenes.
- 污染的hAM样本经过臭氧化动态水的消毒,持续时间不同 (5-15分钟).
- 进行了组织学分析,以评估hAM在臭氧化后的结构变化. 在较长的臭氧化时间 (15-35分钟) 评估了脱氧化.
主要成果:
- 在10分钟内实现了黄金葡萄球菌和Clostridium sporogenes的灭菌.
- 大肠杆菌和葡萄球菌在15分钟内被消毒.
- 坎迪达阿尔比坎斯菌的微生物活性随着暴露时间的延长而下降,但在测试时间内并未完全消除. 在35分钟的臭氧化后,观察到有前途的脱氧化结果.
- 组织学分析显示,表皮质厚度下降,随着臭氧暴露增加而出现一些损伤,这表明可能出现脱表皮化.
结论:
- 臭氧化动态水是一种有效的消毒方法,用于对抗几个常见细菌的hAM.
- 臭氧处理显示了hAM的脱氧化潜力.
- 臭氧暴露可能导致hAM脱皮化,使其成为准备组织工程支架的潜在方法.
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