检测人类黑色素瘤外基因组的协议,这些外基因组会改变人类皮肤细胞的健康表型
Özge Sezin Somuncu1,2,3, Pakize Neslihan Taşli4, Fikrettin Şahin4
1Department of Biotechnology, Yeditepe University, Istanbul, Turkey. ozges_somuncu@dfci.harvard.edu.
Methods in molecular biology (Clifton, N.J.)
|February 27, 2024
概括
携带RAB27a和c-MET蛋白质的黑色素瘤外体可以改变健康细胞. 本研究详细介绍了分析这些癌症衍生的外体和它们对皮肤纤维细胞的影响的方法.
科学领域:
- 细胞生物学 细胞生物学
- 癌症研究 癌症研究
- 细胞外囊泡 细胞外囊泡
背景情况:
- 外体是介导细胞间通信的囊泡,转移像mRNA和蛋白质这样的分子.
- 癌细胞外基因组有助于瘤微环境重编程和疾病进展.
- 黑色素瘤外体内含有特定的蛋白质,包括c-MET和RAB27a,涉及黑色素瘤的进展和外体释放.
研究的目的:
- 为了研究RAB27a和c-MET调节在黑色素瘤外基因组对人类皮肤纤维细胞的影响.
- 分析黑色素瘤衍生的外体细胞在受体细胞中诱导的表型变化.
- 提出针对黑色素瘤外体细胞体内分析的优化方案.
主要方法:
- 从恶性黑色素瘤细胞系中分离出外体.
- 在黑色素瘤细胞中的RAB27a和c-MET表达的下调和上调.
- 用隔离的黑色素瘤外基因组治疗人类皮肤纤维细胞和干细胞.
- 在体外分析受体细胞的表型变化.
主要成果:
- 黑色素瘤外基因组成功地将信息传输到健康的皮肤纤维细胞和干细胞.
- 暴露于黑色素瘤外体引起受体细胞的表型变化.
- 调节RAB27a和c-MET表达影响了外体体载体和受体细胞的反应.
结论:
- 黑色素瘤衍生的外体在细胞间通信中起着重要作用,并且可以诱导非癌细胞的表型变化.
- RAB27a和c-MET是关键蛋白质,参与黑色素瘤外体生物发生和功能.
- 该研究为癌症研究中对外体介导效应的体外调查提供了宝贵的见解和协议.
相关概念视频
Nucleotide Excision Repair
Overview
Nucleotide Excision Repair
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Pigmentation
The color of the skin is influenced by a number of pigments, including melanin, carotene, and hemoglobin. Recall that melanin is produced by cells called melanocytes, which are found scattered throughout the stratum basale of the epidermis. The melanin is transferred to the keratinocytes via melanosomes.
Melanin occurs in two primary forms: eumelanin that provides black and brown pigment and pheomelanin that provides red color. Dark-skinned individuals produce more melanin than those with pale...
Melanin occurs in two primary forms: eumelanin that provides black and brown pigment and pheomelanin that provides red color. Dark-skinned individuals produce more melanin than those with pale...
Changes in Skin Color: Clinical Perspectives
The first thing a clinician sees is the skin, so the examination of the skin should be part of any thorough physical examination. Most skin disorders are relatively benign, but a few, including melanomas, can be fatal if untreated. A couple of the more noticeable disorders, albinism and vitiligo, affect the appearance of the skin and its accessory organs.
Albinism
Albinism is a genetic disorder that affects (completely or partially) the coloring of skin, hair, and eyes. The defect is primarily...
Albinism
Albinism is a genetic disorder that affects (completely or partially) the coloring of skin, hair, and eyes. The defect is primarily...
Skin Diseases and Disorders
Skin is the first line of defense and encounters a variety of microbes. Some pathogenic strains are often the cause of a broad range of infections of the skin and other body systems. These conditions can affect people of all ages and may have different causes, including genetic factors, infections, autoimmune reactions, environmental factors, and lifestyle choices.
Gram-positive Staphylococcus spp. and Streptococcus spp. are responsible for many of the most common skin infections. However, many...
Gram-positive Staphylococcus spp. and Streptococcus spp. are responsible for many of the most common skin infections. However, many...
Skin Cancer
Skin cancer is a type of cancer that occurs when there is an abnormal growth of skin cells, usually triggered by damage to the DNA within the skin cells. It is primarily caused by exposure to ultraviolet (UV) radiation from the sun or artificial sources like tanning beds. Skin cancer is the most common type of cancer worldwide, and its incidence continues to rise.
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...


