蝙蝠中的巴托内拉来自中东的埃及水果蝙蝠
Eva Špitalská1, Martin Ševčík2, Yevheniy-Yuliy Peresh3
1Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Dúbravská Cesta 9, 845 05, Bratislava, Slovakia. eva.spitalska@savba.sk.
Parasitology research
|February 27, 2024
概括
巴托内拉细菌在埃及果蝙蝠中被发现,遍及中东. 这支持了病原体多样性反映了蝙蝠宿主多样性和分化.
科学领域:
- 兽医微生物学 兽医微生物学
- 寄生虫学的寄生虫学
- 蝙蝠生态学蝙蝠生态学
背景情况:
- 巴托内拉菌被检测到在水果蝙蝠 (Pteropodidae) 和它们的外寄生虫在旧世界.
- 在埃及水果蝙蝠 (Rousettus aegyptiacus) 的非洲种群中发现了Candidatus Bartonella rousetti.
- 埃及水果蝙蝠的分布跨越非洲热带和古北极地区,这表明可能存在不同的病原体种群.
研究的目的:
- 为了调查巴托内拉细菌在蝙蝠 (Nycteribiidae) 中的存在和多样性,在中东寄生于埃及果蝙蝠.
- 确定中东地区的巴托内拉菌系是否与同一蝙蝠宿主非洲种群中发现的匹配.
- 探索巴托内拉多样性与宿主蝙蝠的遗传结构之间的关系.
主要方法:
- 在7个中东国家的26个地方从埃及水果蝙蝠 (Rousettus aegyptiacus) 收集蝙蝠 (Eucampsipoda aegyptia).
- 从蝙蝠中提取DNA,然后进行多位置序列分析,针对gltA,ssrA和基因间隔区域 (ITS) 基因.
- 实时PCR用于通过ssrA基因片段的放大来选巴托内拉DNA的存在.
主要成果:
- 来自19个地区的65个Eucampsipoda aegyptia样本检测出巴托内拉DNA呈阳性.
- 在所有七个被调查的国家都发现了巴托内拉阳性蝙蝠.
- 对ITS和gltA基因的序列分析表明,存在属于Cand.的非培养巴顿氏菌物种. B. rousetti基因组,类似于非洲种群中发现的基因组.
结论:
- 这项研究证实了巴托内拉菌在埃及水果蝙蝠外寄生虫中存在.
- 这些发现支持这样一个假设:巴托内拉菌的多样性与其蝙蝠宿主的多样性和遗传结构是一致的.
- 特定的巴托内拉菌系似乎与特定的蝙蝠的基因组有关.
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